Kühn I, Brauner A, Möllby R
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Epidemiol Infect. 1990 Dec;105(3):521-31. doi: 10.1017/s0950268800048147.
Reproducible and discriminating typing methods are required for epidemiological investigations. Numerical typing systems analyse patterns obtained in various ways by calculating similarity coefficients between isolates. In the present study, various measures of the efficiency of a numerical typing system are quantified. These include reproducibility, accuracy, and discrimination power. Three different numerical typing methods for Escherichia coli were compared using these measures: (a) Biotyping with API 50 CH system, (b) Biochemical fingerprinting with the API 50 CH system and (c) Biochemical fingerprinting with the PhP-EC system. Biotyping qualitatively measures the results of a set of biochemical reactions as + or -. Biochemical fingerprinting also uses biochemical reactions, but the tests are scored quantitatively by measuring the kinetics and intensity of each reaction. It was found that biotyping yielded poor reproducibility. When biochemical fingerprinting analysis was used with the API 50 CHE system the reproducibility and the discrimination was good. The PhP-EC system for biochemical fingerprinting showed equal reproducibility but was superior to the API 50 CH system with regard to discrimination power.
流行病学调查需要可重复且具有区分能力的分型方法。数字分型系统通过计算分离株之间的相似系数来分析以各种方式获得的模式。在本研究中，对数字分型系统的各种效率指标进行了量化。这些指标包括可重复性、准确性和区分能力。使用这些指标比较了三种不同的大肠杆菌数字分型方法：(a) 使用API 50 CH系统进行生物分型，(b) 使用API 50 CH系统进行生化指纹图谱分析，以及(c) 使用PhP-EC系统进行生化指纹图谱分析。生物分型以定性方式将一组生化反应的结果记为 + 或 -。生化指纹图谱分析也使用生化反应，但通过测量每个反应的动力学和强度对测试进行定量评分。结果发现，生物分型的可重复性较差。当使用API 50 CHE系统进行生化指纹图谱分析时，可重复性和区分能力良好。用于生化指纹图谱分析的PhP-EC系统显示出相同的可重复性，但在区分能力方面优于API 50 CH系统。
