Unosson Erik, Cai Yanling, Jiang Xiyuan, Lööf Jesper, Welch Ken, Engqvist Håkan
Division of Applied Materials Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, Sweden.
Int J Dent. 2012;2012:529495. doi: 10.1155/2012/529495. Epub 2012 Mar 14.
A modified direct contact test was used to evaluate the antibacterial properties of four commercially available dental luting agents (RelyX Unicem, Ketac Cem, Ceramir Crown & Bridge and Harvard Cement) and two reference materials (glass-ionomer cement and calcium aluminate cement) compared to a negative-control material (PMMA). Streptococcus mutans bacteria were placed in direct contact with specimens that had been aged for 10 min, 1 day, and 7 days, in order to test the antibacterial properties of the materials. A metabolic assay containing resazurin was used to quantify the amount of viable bacteria remaining after the direct contact tests. The effects of pH and fluoride on bacteria proliferation were also evaluated. Strongest antibacterial properties were found for calcium aluminate cement, followed by Ceramir Crown & Bridge and RelyX Unicem. Ketac Cem, Harvard Cement, and the reference glass-ionomer cement showed bacteria content either higher than or not significantly different from the PMMA control in all instances. pH levels below 6.3 and above 9.0 were found to have negative effects on bacterial proliferation. No correlation between either acidic materials or fluoride release and antibacterial properties could be seen; rather, basic materials showed stronger antibacterial properties.
采用改良的直接接触试验，与阴性对照材料（聚甲基丙烯酸甲酯，PMMA）相比，评估四种市售牙科粘结剂（瑞速齐通用型粘结剂、卡塔Cem、Ceramir冠桥专用粘结剂和哈佛水泥）以及两种参比材料（玻璃离子水门汀和铝酸钙水泥）的抗菌性能。将变形链球菌与分别老化10分钟、1天和7天的样本直接接触，以测试材料的抗菌性能。采用含刃天青的代谢试验来量化直接接触试验后残留的活菌数量。还评估了pH值和氟对细菌增殖的影响。发现铝酸钙水泥的抗菌性能最强，其次是Ceramir冠桥专用粘结剂和瑞速齐通用型粘结剂。在所有情况下，卡塔Cem、哈佛水泥和参比玻璃离子水门汀的细菌含量均高于PMMA对照组或与PMMA对照组无显著差异。发现pH值低于6.3和高于9.0对细菌增殖有负面影响。未观察到酸性材料或氟释放与抗菌性能之间存在相关性；相反，碱性材料显示出更强的抗菌性能。
