School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA.
Opt Lett. 2012 Apr 15;37(8):1349-51. doi: 10.1364/OL.37.001349.
We report a miniaturized resonant/non-resonant multi-fiber raster scanner that is paired with a gradient-index lens assembly to achieve a compact and flexible multifocal multiphoton endoscope capable of longitudinal parallel image acquisition. Multiphoton images are obtained simultaneously at three axial depths, separated by ≥4.8 μm, by incorporating three axially offset double clad optical fibers into the miniaturized scanner. The fabricated endoscope has an outer diameter of 3 mm, a rigid length of 4 cm, and acquires images at 4 frames/s per focal plane, with lateral and axial resolutions for two-photon imaging of 0.8 and 10 μm, respectively.
我们报告了一种小型化的共振/非共振多光纤光栅扫描仪，它与梯度折射率透镜组件配对，以实现紧凑灵活的多焦点多光子内窥镜，能够进行纵向并行图像采集。通过将三根轴向偏移的双包层光纤集成到小型化扫描仪中，可在三个轴向深度处同时获得多光子图像，深度间隔≥4.8μm。所制造的内窥镜外径为 3 毫米，刚性长度为 4 厘米，每个焦平面以 4 帧/秒的速度采集图像，双光子成像的横向和轴向分辨率分别为 0.8 和 10 微米。
