Fabbri R, Vicenti R, Macciocca M, Pasquinelli G, Lima M, Parazza I, Magnani V, Venturoli S
Gynecology and Reproductive Medicine Unit, Department of Obstetrics and Gynecology, S. Orsola-Malpighi Hospital, Bologna 40138, Italy.
Obstet Gynecol Int. 2012;2012:910698. doi: 10.1155/2012/910698. Epub 2012 Feb 13.
Cancer treatments improve the survival rate of children and adolescents; however chemo- and radiotherapy result in gonadal damage leading to acute ovarian failure and sterility. Ovarian tissue cryopreservation allows long-term storage of primordial follicles and represents the only possibility of preserving the potential fertility in prepubertal girls. The aim of the present study is to describe our experience in ovarian tissue cryopreservation in 45 pediatric patients. The number of follicles per square millimeter of the overall section area and follicle quality were evaluated histologically. A strong negative correlation was found between age and follicular density in patients both prior to and after chemotherapy (P < 0.0001). Damage in follicular quality, that is, increased oocyte vacuolization and detachment of the oocyte from granulosa cells, was found after chemotherapy. Ovarian tissue cryopreservation, preferably performed before initiation of chemotherapy, should be offered to pediatric patients, including prepubertal girls, at risk of sterility.
癌症治疗提高了儿童和青少年的生存率；然而，化疗和放疗会导致性腺损伤，进而引发急性卵巢功能衰竭和不育。卵巢组织冷冻保存可以长期储存原始卵泡，是青春期前女孩保留潜在生育能力的唯一方法。本研究的目的是描述我们在45例儿科患者中进行卵巢组织冷冻保存的经验。通过组织学评估每平方毫米整个切片区域的卵泡数量和卵泡质量。化疗前后患者的年龄与卵泡密度之间均存在显著的负相关（P < 0.0001）。化疗后发现卵泡质量受损，即卵母细胞空泡化增加以及卵母细胞与颗粒细胞分离。对于有不育风险的儿科患者，包括青春期前女孩，应提供卵巢组织冷冻保存，最好在化疗开始前进行。
