Kim Young-Ok, Park In-Suk, Park Sooyeon, Nam Bo-Hye, Kim Dong-Gyun, Choi Seok-Gwan, Bae Jin-Woo, Yoon Jung-Hoon
Biotechnology Research Division, National Institute of Fisheries Science (NIFS), Gijang, Busan 46083, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1227-1232. doi: 10.1099/ijsem.0.002656. Epub 2018 Feb 22.
A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterial strain, designated RA3-3-1, was isolated from splendid alfonsino (Beryxsplendens) collected from the North Pacific Ocean. Strain RA3-3-1 grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA3-3-1 belonged to the genus Bizionia, clustering with the type strain of Bizionia fulviae. Strain RA3-3-1 exhibited 16S rRNA gene sequence similarities of 98.7, 97.6 and 97.3 % to the type strains of B. fulviae, Bizionia paragorgiae and Bizionia saleffrena, respectively, and of 95.5-96.4 % to the type strains of the other Bizionia species. Strain RA3-3-1 contained MK-6 as the predominant menaquinone and anteiso-C15 : 0, iso-C16 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, iso-C15 : 0 and C17 : 0 2-OH as the major fatty acids. The major polar lipids detected in strain RA3-3-1 were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain RA3-3-1 was 34.1 mol% and its DNA-DNA relatedness values with the type strains of B. fulviae, B. paragorgiae and B. saleffrena were 12-29 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RA3-3-1 is separate from recognized species of the genus Bizionia. On the basis of the data presented, strain RA3-3-1 is considered to represent a novel species of the genus Bizionia, for which the name Bizionia berychis sp. nov. is proposed. The type strain is RA3-3-1 (=KCTC 62140=NBRC 113024).
从北太平洋采集的红金眼鲷（Beryx splendens）中分离出一株革兰氏阴性、无运动性、需氧的杆状细菌，命名为RA3-3-1。RA3-3-1菌株在25 °C、pH 7.0 - 8.0以及1.0 - 3.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树显示，RA3-3-1菌株属于Bizionia属，与富氏Bizionia fulviae的模式菌株聚类。RA3-3-1菌株与富氏B. fulviae、拟深海Bizionia paragorgiae和盐沼Bizionia saleffrena的模式菌株的16S rRNA基因序列相似性分别为98.7%、97.6%和97.3 %，与其他Bizionia属模式菌株的相似性为95.5 - 96.4 %。RA3-3-1菌株以MK-6作为主要甲基萘醌，以anteiso-C15 : 0、iso-C16 : 0 3-OH、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、iso-C17 : 0 3-OH、iso-C15 : 0和C17 : 0 2-OH作为主要脂肪酸。在RA3-3-1菌株中检测到的主要极性脂类为磷脂酰乙醇胺、一种未鉴定的脂类和一种未鉴定的氨基脂类。RA3-3-1菌株的DNA G+C含量为34.1 mol%，其与富氏B. fulviae、拟深海B. paragorgiae和盐沼B. saleffrena模式菌株的DNA-DNA相关性值为12 - 29 %。不同的表型特征，以及其系统发育和遗传独特性，表明RA3-3-1菌株与已确认的Bizionia属物种不同。基于所提供的数据，RA3-3-1菌株被认为代表Bizionia属的一个新物种，为此提出名为Bizionia berychis sp. nov.的名称。模式菌株为RA3-3-1（=KCTC 62140=NBRC 113024）。
