Bonami J R, Trumper B, Mari J, Brehelin M, Lightner D V
ERPAM, Laboratoire de Pathologie Comparée, INRA-CNRS, Université des Sciences et Techniques du Languedoc, Montpellier, France.
J Gen Virol. 1990 Nov;71 ( Pt 11):2657-64. doi: 10.1099/0022-1317-71-11-2657.
Infectious hypodermal and haematopoietic necrosis (IHHN) is one of the most important viral diseases of cultured penaeid shrimps and is potentially a limiting factor in the development of farming projects for some species of these shrimps. Although the IHHN agent was recognized early as being viral in origin, attempts to characterize it were inconclusive because of difficulties in obtaining sufficient amounts of purified virions to permit its characterization. Recent improvements of purification procedures have allowed the physicochemical characterization of this virus. Purified IHHNV is a non-enveloped icosahedral particle averaging 22 nm in diameter, exhibiting a mean buoyant density of 1.40 g/ml in CsCl. The genome is a single molecule of ssDNA with an estimated size of 4.1 kb by molecule length measurement in transmission electron microscopy. As determined by SDS-PAGE, the particle contains four polypeptides with Mrs of 74K, 47K, 39K and 37.5K, respectively. From its characteristics, this virus could be a member of the Parvoviridae family.
传染性皮下和造血组织坏死病（IHHN）是养殖对虾最重要的病毒性疾病之一，并且可能是某些对虾养殖项目发展的限制因素。尽管IHHN病原体很早就被认为起源于病毒，但由于难以获得足够量的纯化病毒粒子以进行特性鉴定，对其进行特性描述的尝试没有定论。最近纯化程序的改进使得对该病毒进行物理化学特性鉴定成为可能。纯化的IHHNV是一种无包膜的二十面体颗粒，平均直径为22nm，在CsCl中的平均浮力密度为1.40g/ml。通过透射电子显微镜测量分子长度，基因组是一个单链DNA分子，估计大小为4.1kb。通过SDS-PAGE测定，该颗粒分别包含四种分子量为74K、47K、39K和37.5K的多肽。从其特性来看，这种病毒可能是细小病毒科的成员。
