Lakeman A D, Osborn J E
J Virol. 1979 Apr;30(1):414-6. doi: 10.1128/JVI.30.1.414-416.1979.
Viral DNA was isolated from human and murine cytomegalovirus by equilibrium centrifugation in cesium chloride gradients. The size of the DNA was measured relative to T4 DNA by velocity sedimentation in neutral glycerol gradients, and fractions were assayed for infectious DNA. Infectious murine cytomegalovirus DNA sedimented as a single peak with an estimated molecular weight of 136 X 10(6). Infectious human cytomegalovirus DNA was detected in two peaks with molecular weights of 130 X 10(6) and 150 X 10(6).
通过在氯化铯梯度中进行平衡离心，从人巨细胞病毒和鼠巨细胞病毒中分离出病毒DNA。通过在中性甘油梯度中进行速度沉降，相对于T4 DNA测量DNA的大小，并对各组分进行感染性DNA检测。感染性鼠巨细胞病毒DNA以单峰形式沉降，估计分子量为136×10⁶。在分子量为130×10⁶和150×10⁶的两个峰中检测到感染性人巨细胞病毒DNA。
