Chiu M-H, Su C-L, Chen C-F, Chen K-H, Wang D, Wang J-J
Chest Department, Cathay General Hospital, Taipei, Taiwan.
Transplant Proc. 2012 May;44(4):962-5. doi: 10.1016/j.transproceed.2012.01.097.
Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability.
Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (K(fc)), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury.
Liver I/R-induced lung injury was noted by the markedly increased K(fc), W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased (P < .05). All indicators were markedly decreased in melatonin-pretreated rats (P < .05), suggesting that lung injury was attenuated.
Melatonin pretreatment prior to liver I/R can effectively reduce the pulmonary microvascular permeability and attenuate lipid peroxidation in the lungs.
肝脏再灌注过程中产生的活性氧与远隔肺损伤有关。在本研究中，我们评估褪黑素预处理对肺微血管通透性增加的保护作用。
雄性Sprague-Dawley大鼠分为三组：假手术组、肝脏缺血再灌注（I/R）组和在肝脏I/R前15分钟腹腔注射褪黑素预处理（15mg/kg）组。缺血持续30分钟，随后再灌注2小时。原位分离肺脏，采用毛细血管滤过系数（K(fc)）、肺湿重与干重比值（W/D）、肺重与体重比值（LW/BW）、支气管肺泡灌洗液蛋白浓度（PCBAL）、支气管肺泡灌洗液（BALF）中巨噬细胞和中性粒细胞百分比以及肺组织丙二醛等参数评估肺损伤。
肝脏I/R诱导的肺损伤表现为K(fc)、W/D、LW/BW、PCBAL显著增加，BALF中出现中性粒细胞和巨噬细胞。脂质过氧化也增加（P<.05）。褪黑素预处理大鼠的所有指标均显著降低（P<.05），表明肺损伤减轻。
肝脏I/R前进行褪黑素预处理可有效降低肺微血管通透性并减轻肺组织脂质过氧化。
