通过流动注射化学发光法测定酪氨酸。

Determination of tyrosine through a FIA-direct chemiluminescence procedure.

机构信息

Departamento de Quimica, Bioquimica y Biologia Molecular, Universidad Cardenal Herrera - C.E.U., 46113 Moncada, Valencia, Spain.

出版信息

Talanta. 2003 Jun 13;60(2-3):369-76. doi: 10.1016/S0039-9140(03)00099-7.

DOI:10.1016/S0039-9140(03)00099-7

PMID:18969059

Abstract

A new FI-direct chemiluminescence method is proposed for the determination of tyrosine, based on the oxidation of the amino acid by K(3)Fe(CN)(6) in potassium hydroxide medium, at room temperature and enhanced by the presence of beta-cyclodextrin and formic acid. The dynamic range was linear over the range 1.0-10.0 mgl(-1). A large study of the influence of foreign compounds was performed, including amino acids; and, the method showed high selectivity. The reproducibility between days resulted in a rsd (in slope%) of 4.8 and the repeatability with a rsd (n=50, 10.0 mgl(-1)) of 3.1%, the LOD (s/n=3) was 50 mugl(-1) and sample throughput 98 h(-1).

摘要

提出了一种新的 FI 直接化学发光法，用于测定酪氨酸，基于氨基酸在室温下被 K(3)Fe(CN)(6)氧化，在碱性介质中，在β-环糊精和甲酸的存在下增强。在 1.0-10.0 mgl(-1)的范围内呈现出线性范围。对外来化合物的影响进行了大量研究，包括氨基酸；该方法表现出高选择性。在不同天之间的重现性导致斜率的 rsd（%）为 4.8，而在 10.0 mgl(-1)时的重复性的 rsd（n=50）为 3.1%，LOD（s/n=3）为 50 mugl(-1)，样品通量为 98 h(-1)。