通过放射免疫测定法比较不同狂犬病病毒血清型的核糖核蛋白

Comparison of the ribonucleoproteins of different rabies virus serotypes by radioimmunoassay.

作者信息

Bruns M, Dietzschold B, Schneider L G, Cox J H

出版信息

J Immunol Methods. 1977;18(3-4):337-46. doi: 10.1016/0022-1759(77)90187-9.

Abstract

Radioimmunoassay (RIA) provides a sensitive serological procedure for detecting rabies virus ribonucleoprotein (RNP) as well as its specific antibodies, RIA was carried out using highly purified RNPs labelled by the chloramine-T method. This paper describes optimal conditions for iodination of RNP with high specific activity. The optimal concentrations of 125I, RNP, chloramine-T, and reducing agent as well as the effect of pH on the reaction were investigated. RIA proved to be extremely sensitive for detection of homologous antibodies. In competition experiments the part-relationship of the group-specific RNPs of the three rabies virus serotypes (HEP, MOK and LBV) was confirmed.

摘要

放射免疫测定法（RIA）为检测狂犬病病毒核糖核蛋白（RNP）及其特异性抗体提供了一种灵敏的血清学方法。使用氯胺-T法标记的高度纯化的核糖核蛋白进行放射免疫测定。本文描述了用高比活性对核糖核蛋白进行碘化的最佳条件。研究了125I、核糖核蛋白、氯胺-T和还原剂的最佳浓度以及pH对反应的影响。放射免疫测定法被证明对检测同源抗体极为灵敏。在竞争实验中，证实了三种狂犬病病毒血清型（HEP、MOK和LBV）的群特异性核糖核蛋白的部分关系。