人IgA Fc受体的表达克隆
Expression cloning of a human Fc receptor for IgA.
作者信息
Maliszewski C R, March C J, Schoenborn M A, Gimpel S, Shen L
机构信息
Immunex Corporation, Seattle, Washington 98101.
出版信息
J Exp Med. 1990 Dec 1;172(6):1665-72. doi: 10.1084/jem.172.6.1665.
Abstract
IgA, the predominant isotype in secretions, mediates the neutralization and removal of environmental antigens from mucosal sites. Although cell surface receptors for the Fc region of IgA (Fc alpha R) have been implicated in a variety of immune effector mechanisms, the molecular features of Fc alpha R remain only marginally characterized. In this report, we describe the isolation of a clone from a myeloid cell line cDNA library that directs the expression of a cell surface molecule with IgA binding specificity. The cDNA encodes a peptide of Mr 30,000 including a putative transmembrane region with features atypical of conventional membrane-anchored proteins. Databank searches indicate that the human myeloid cell Fc alpha R sequence is unique, is a member of the immunoglobulin gene superfamily, and is related to Fc receptors for IgG (Fc gamma RI, II, and III) and IgE (Fc epsilon RI).
摘要
IgA是分泌物中的主要同种型，介导从黏膜部位中和并清除环境抗原。尽管IgA的Fc区细胞表面受体（FcαR）参与了多种免疫效应机制，但FcαR的分子特征仍未得到充分表征。在本报告中，我们描述了从髓样细胞系cDNA文库中分离出一个克隆，该克隆指导表达具有IgA结合特异性的细胞表面分子。该cDNA编码一个30000 Mr的肽，包括一个具有非传统膜锚定蛋白特征的假定跨膜区域。数据库搜索表明，人类髓样细胞FcαR序列是独特的，是免疫球蛋白基因超家族的成员，并且与IgG（FcγRI、II和III）和IgE（FcεRI）的Fc受体相关。
相似文献
1
Expression cloning of a human Fc receptor for IgA.人IgA Fc受体的表达克隆
J Exp Med. 1990 Dec 1;172(6):1665-72. doi: 10.1084/jem.172.6.1665.
2
Characterization and expression of an Fc gamma receptor cDNA cloned from rat natural killer cells.从大鼠自然杀伤细胞克隆的Fcγ受体cDNA的特性与表达
Proc Natl Acad Sci U S A. 1990 May;87(9):3425-9. doi: 10.1073/pnas.87.9.3425.
3
Molecular structure and expression of the murine lymphocyte low-affinity receptor for IgE (Fc epsilon RII).小鼠IgE低亲和力受体（FcεRII）的分子结构与表达
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7566-70. doi: 10.1073/pnas.86.19.7566.
4
Cloning of cDNA coding for low-affinity Fc receptors for IgE on human T lymphocytes.
Int Immunol. 1989;1(3):254-9. doi: 10.1093/intimm/1.3.254.
5
Structure of the gene for the human myeloid IgA Fc receptor (CD89).人类髓系IgA Fc受体（CD89）的基因结构。
J Immunol. 1995 Aug 1;155(3):1203-9.
6
Characterization and expression of the gene for the human Fc receptor gamma subunit. Definition of a new gene family.人Fc受体γ亚基基因的表征与表达。一个新基因家族的定义。
J Biol Chem. 1990 Apr 15;265(11):6448-52.
7
Cloning and expression of the cDNA coding for a human lymphocyte IgE receptor.编码人淋巴细胞IgE受体的cDNA的克隆与表达
EMBO J. 1987 Jan;6(1):109-14. doi: 10.1002/j.1460-2075.1987.tb04726.x.
8
Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII).编码人IgG受体（FcγRII）的cDNA克隆的分离与表达
J Exp Med. 1987 Dec 1;166(6):1668-84. doi: 10.1084/jem.166.6.1668.
9
10
Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.人FcγRIII：两种IgG Fc受体的克隆、表达及染色体定位鉴定
Proc Natl Acad Sci U S A. 1989 Feb;86(3):1013-7. doi: 10.1073/pnas.86.3.1013.
引用本文的文献
1
A genetically determined molecular switch modulates the anti-inflammatory potential of human IgA.一种由基因决定的分子开关调节人类免疫球蛋白A的抗炎潜能。
Front Immunol. 2025 Aug 27;16:1641351. doi: 10.3389/fimmu.2025.1641351. eCollection 2025.
2
Interaction study of the effects of environmental exposure and gene polymorphisms of inflammatory and immune-active factors on chronic obstructive pulmonary disease.环境暴露及炎症与免疫活性因子基因多态性对慢性阻塞性肺疾病影响的交互作用研究
Respir Res. 2025 Jan 8;26(1):5. doi: 10.1186/s12931-024-03079-4.
3
FcαRI (CD89) is upregulated on subsets of mucosal and circulating NK cells and regulates IgA-class specific signaling and functions.FcαRI（CD89）在黏膜和循环 NK 细胞的亚群上上调，并调节 IgA 类特异性信号转导和功能。
Mucosal Immunol. 2024 Aug;17(4):692-699. doi: 10.1016/j.mucimm.2024.04.003. Epub 2024 Apr 25.
4
Fc receptors act as innate immune receptors during infection?Fc 受体在感染期间作为先天免疫受体发挥作用？
Front Immunol. 2023 Jul 26;14:1188497. doi: 10.3389/fimmu.2023.1188497. eCollection 2023.
5
The role of mononuclear phagocyte system in IgA nephropathy: pathogenesis and prognosis.单核吞噬细胞系统在 IgA 肾病中的作用：发病机制与预后。
Front Immunol. 2023 Jul 3;14:1192941. doi: 10.3389/fimmu.2023.1192941. eCollection 2023.
6
Serological Measurement of Poly-IgA Immune Complex Levels in IgA Nephropathy and IgA Vasculitis.IgA肾病和IgA血管炎中多聚IgA免疫复合物水平的血清学检测
Bio Protoc. 2022 Jul 5;12(13). doi: 10.21769/BioProtoc.4463.
7
Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and L.影响IgA1和IgA2与抗原、FcαRI以及超抗原蛋白G、A和L结合的可变重链（VH）家族。
Sci Rep. 2022 Apr 20;12(1):6510. doi: 10.1038/s41598-022-10388-5.
8
Crimean-Congo Hemorrhagic Fever Virus Past Infections Are Associated with Two Innate Immune Response Candidate Genes in Dromedaries.克里米亚-刚果出血热病毒既往感染与单峰驼中两个先天免疫反应候选基因相关。
Cells. 2021 Dec 21;11(1):8. doi: 10.3390/cells11010008.
9
Fc Receptor Variants and Disease: A Crucial Factor to Consider in the Antibody Therapeutics in Clinic.Fc 受体变异体与疾病：抗体治疗药物在临床应用中需要考虑的关键因素。
Int J Mol Sci. 2021 Aug 31;22(17):9489. doi: 10.3390/ijms22179489.
10
Recent Advances in Allergy Research Using Humanized Mice.利用人源化小鼠进行过敏研究的最新进展。
Int J Mol Sci. 2019 Jun 4;20(11):2740. doi: 10.3390/ijms20112740.
本文引用的文献
1
Receptors for IgA on human lymphocytes--I. detection and specificity.人淋巴细胞上IgA的受体——I. 检测与特异性
Mol Immunol. 1981 Mar;18(3):189-95. doi: 10.1016/0161-5890(81)90085-7.
2
Secretory IgA antibodies synergize with IgG in promoting ADCC by human polymorphonuclear cells, monocytes, and lymphocytes.分泌型IgA抗体与人多形核细胞、单核细胞及淋巴细胞协同作用，促进抗体依赖的细胞介导的细胞毒性作用（ADCC）。
Cell Immunol. 1981 Mar 15;59(1):75-81. doi: 10.1016/0008-8749(81)90435-4.
3
Subpopulations of human peripheral granulocyes and monocytes express receptors for IgA.人类外周粒细胞和单核细胞的亚群表达IgA受体。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3640-4. doi: 10.1073/pnas.77.6.3640.
4
Establishing homologies in protein sequences.确定蛋白质序列中的同源性。
Methods Enzymol. 1983;91:524-45. doi: 10.1016/s0076-6879(83)91049-2.
5
Fc receptors for IgA and other immunoglobulins on resident and activated alveolar macrophages.驻留和活化肺泡巨噬细胞上的IgA及其他免疫球蛋白的Fc受体
Mol Immunol. 1983 Sep;20(9):1029-37. doi: 10.1016/0161-5890(83)90044-5.
6
Cytofluorographic analysis of receptors for IgA on human polymorphonuclear cells and monocytes and the correlation of receptor expression with phagocytosis.人多形核细胞和单核细胞上IgA受体的细胞荧光分析以及受体表达与吞噬作用的相关性。
Mol Immunol. 1983 Sep;20(9):1019-27. doi: 10.1016/0161-5890(83)90043-3.
7
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
8
The Alu family of dispersed repetitive sequences.分散重复序列的Alu家族。
Science. 1982 Jun 4;216(4550):1065-70. doi: 10.1126/science.6281889.
9
Production of target-specific effector cells using hetero-cross-linked aggregates containing anti-target cell and anti-Fc gamma receptor antibodies.使用含有抗靶细胞抗体和抗Fcγ受体抗体的异源交联聚集体产生靶向特异性效应细胞。
J Exp Med. 1984 Dec 1;160(6):1686-701. doi: 10.1084/jem.160.6.1686.
10
A simple and very efficient method for generating cDNA libraries.一种简单且非常有效的生成cDNA文库的方法。
Gene. 1983 Nov;25(2-3):263-9. doi: 10.1016/0378-1119(83)90230-5.
Zotero 插件