对巴西健康女性和感染女性阴道乳酸杆菌的分析。
Analysis of vaginal lactobacilli from healthy and infected Brazilian women.
作者信息
Martinez Rafael C R, Franceschini Sílvio A, Patta Maristela C, Quintana Silvana M, Nunes Alvaro C, Moreira João L S, Anukam Kingsley C, Reid Gregor, De Martinis Elaine C P
机构信息
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
出版信息
Appl Environ Microbiol. 2008 Jul;74(14):4539-42. doi: 10.1128/AEM.00284-08. Epub 2008 May 23.
Abstract
Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC.
摘要
采用基于培养的聚合酶链反应（PCR）扩增rRNA基因限制性分析和非培养方法（PCR-变性梯度凝胶电泳），检测巴西健康女性或已诊断为外阴阴道念珠菌病（VVC）或细菌性阴道病的女性的阴道乳酸杆菌。两种方法均仅相应地检测到卷曲乳酸杆菌，且产H₂O₂的乳酸杆菌与预防VVC无关。
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