State Key Laboratory of Organic Geochemistry, Guangzhou Geochemistry Institute of Chinese Academy of Sciences, Guangzhou 510640, China.
Talanta. 2012 May 30;94:232-9. doi: 10.1016/j.talanta.2012.03.029. Epub 2012 Mar 21.
A simple, reliable and sensitive method for the simultaneous determination of caffeic acid phenethyl ester (CAPE) and its metabolite caffeic acid (CA) in dog plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS). The sample pretreatment generally involved protein precipitation treatment (PPT) and direct dilution. CAPE and CA were separated with a C18 reversed-phase column. Electrospray ionization (ESI) interface operated in negative mode was chosen for ionization. Multiple reaction monitoring (MRM) mode was selected for data acquisition. The quantification range was 10.0-10,000.0 ng mL(-1). The intra- and inter-batch accuracies were within 92.5-107.0% with relative standard deviation (RSD, %) no more than 10.5%. CAPE and CA were proved to be stable in stabilizer-treated dog blood and PPT-treated plasma during the sampling and pretreatment period. The applicability has been evaluated with real samples from treated dogs.
建立了一种同时测定犬血浆中咖啡酸苯乙酯（CAPE）及其代谢物咖啡酸（CA）的液相色谱-串联质谱（LC-MS/MS）分析方法。样品预处理一般采用蛋白沉淀（PPT）和直接稀释法。CAPE 和 CA 采用 C18 反相柱分离，电喷雾离子源（ESI）在负离子模式下进行离子化，多反应监测（MRM）模式进行数据采集。定量范围为 10.0-10,000.0ng·mL-1。内、日间精密度均在 92.5%-107.0%范围内，相对标准偏差（RSD）不超过 10.5%。CAPE 和 CA 在稳定剂处理的犬血和 PPT 处理的血浆中具有良好的稳定性，在采样和预处理期间均稳定。已用经处理犬的实际样品对该方法的适用性进行了评价。
