Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy.
J Proteomics. 2012 Jul 16;75(13):4050-61. doi: 10.1016/j.jprot.2012.05.019. Epub 2012 May 23.
Lactobacillus plantarum is a facultative heterofermentative lactic acid bacterium widely used in the production of most fermented food due to its ability to thrive in several environmental niches, including the human gut. In order to cope with different growth conditions, it has developed complex molecular response mechanisms, characterized by the induction of a large set of proteins mainly regulated by HrcA and CtsR repressors as well as by global regulators such as carbon catabolite control protein A (CcpA). In this study, the role of CcpA in the regulation of growth under anaerobiosis and aerobiosis, and the adaptation to aeration in L. plantarum WCFS1 were comprehensively investigated by differential proteomics. The inactivation of ccpA, in both growth conditions, significantly changed the expression level of 76 proteins, mainly associated with carbohydrate and energy metabolism, membrane transport, nucleotide metabolism, protein biosynthesis and folding. The role of CcpA as pleiotropic regulator was particularly evident at the shift from homolactic fermentation to mixed fermentation. Proteomic results also indicated that the mutant strain was more responsive to aerobic growth condition.
植物乳杆菌是一种兼性异型发酵乳酸菌，由于其能够在包括人类肠道在内的多个环境小生境中茁壮成长，因此广泛用于大多数发酵食品的生产。为了应对不同的生长条件，它已经发展出了复杂的分子响应机制，其特征是诱导大量主要受 HrcA 和 CtsR 抑制剂以及全局调节剂（如碳分解代谢物控制蛋白 A（CcpA））调节的蛋白质。在这项研究中，通过差异蛋白质组学全面研究了 CcpA 在厌氧和需氧生长条件下的调节作用，以及在植物乳杆菌 WCFS1 中对通气的适应作用。在两种生长条件下，ccpA 的失活都会显著改变 76 种蛋白质的表达水平，这些蛋白质主要与碳水化合物和能量代谢、膜转运、核苷酸代谢、蛋白质生物合成和折叠有关。CcpA 作为多效调节剂的作用在从同质乳酸发酵到混合发酵的转变中尤为明显。蛋白质组学结果还表明，突变株对有氧生长条件的反应更为敏感。
