Australian Proteome Analysis Facility, Macquarie University, Sydney NSW 2109, Australia.
J Proteomics. 2012 Aug 3;75(15):4802-19. doi: 10.1016/j.jprot.2012.05.018. Epub 2012 May 24.
Cytokines, chemokines, growth factors (CCGFs) and other low abundance proteins/peptides in human body fluids or in tissues are potential biomarkers. Human body fluids such as plasma, saliva, urine, etc. are being analyzed more frequently than tissues primarily because of ease of sample collection. However, available information on concentrations of a large number of CCGFs in various body fluids of the same healthy individuals and gender-specific CCGFs is limited. In this work concentrations of 48 CCGFs were measured using multiplex bead assays and compared between plasma, saliva and urine collected from 20 male and female healthy volunteers. Forty three CCGFs were detected at least in one sample type of which 37 were in plasma, 41 were in saliva, and 34 were in urine; five CCGFs were not detected in any sample. Concentrations of detected CCGFs differed significantly between sample types but similar between gender groups. Gender-specific CCGFs were also observed. Concentrations of nine acute phase proteins were also measured from plasma, saliva and urine to determine general health conditions of the volunteers. This work will provide an idea of which CCGFs are detectable and their relative concentrations in healthy human plasma, saliva and urine and which CCGFs are gender-specific.
细胞因子、趋化因子、生长因子（CCGFs）和其他在人体体液或组织中低丰度的蛋白质/肽类是潜在的生物标志物。人体体液如血浆、唾液、尿液等，由于样本采集较为容易，因此比组织更常被分析。然而，关于大量 CCGFs 在同健康个体的各种体液中的浓度以及性别特异性 CCGFs 的信息有限。在这项工作中，使用多重微球检测法测量了 48 种 CCGFs，并比较了从 20 名男性和女性健康志愿者采集的血浆、唾液和尿液中的浓度。至少在一种样本类型中检测到 43 种 CCGFs，其中 37 种存在于血浆中，41 种存在于唾液中，34 种存在于尿液中；有 5 种 CCGFs在任何样本中都未被检测到。检测到的 CCGFs 的浓度在样本类型之间有显著差异，但在性别组之间相似。还观察到了性别特异性的 CCGFs。也从血浆、唾液和尿液中测量了 9 种急性期蛋白的浓度，以确定志愿者的一般健康状况。这项工作将提供一个概念，即哪些 CCGFs是可检测的，以及它们在健康人体血浆、唾液和尿液中的相对浓度，以及哪些 CCGFs是性别特异性的。
