Energy Biosciences Institute, University of California Berkeley, CA, USA.
Front Plant Sci. 2012 May 11;3:89. doi: 10.3389/fpls.2012.00089. eCollection 2012.
Plant cell walls are composed of interlinked polymer networks consisting of cellulose, hemicelluloses, pectins, proteins, and lignin. The ordered deposition of these components is a dynamic process that critically affects the development and differentiation of plant cells. However, our understanding of cell wall synthesis and remodeling, as well as the diverse cell wall architectures that result from these processes, has been limited by a lack of suitable chemical probes that are compatible with live-cell imaging. In this review, we summarize the currently available molecular toolbox of probes for cell wall polysaccharide imaging in plants, with particular emphasis on recent advances in small molecule-based fluorescent probes. We also discuss the potential for further development of small molecule probes for the analysis of cell wall architecture and dynamics.
植物细胞壁由纤维素、半纤维素、果胶、蛋白质和木质素等聚合物网络相互连接而成。这些成分的有序沉积是一个动态过程，对植物细胞的发育和分化起着至关重要的作用。然而，由于缺乏与活细胞成像兼容的合适化学探针，我们对细胞壁合成和重塑以及由此产生的不同细胞壁结构的理解一直受到限制。在这篇综述中，我们总结了目前可用于植物细胞壁多糖成像的分子工具包，特别强调了基于小分子的荧光探针的最新进展。我们还讨论了进一步开发小分子探针用于分析细胞壁结构和动态的潜力。
