Department of Chemistry and Biochemistry and the Center for Nanoscience, University of Missouri-St. Louis, One University Boulevard, St. Louis, Missouri 63121, USA.
Org Lett. 2012 Jun 15;14(12):3036-9. doi: 10.1021/ol301105y. Epub 2012 May 30.
A standard HPLC was adapted to polymer supported oligosaccharide synthesis. Solution-based reagents are delivered using a software-controlled solvent delivery system. The reaction progress and completion can be monitored in real time using a standard UV detector. All steps of oligosaccharide assembly including loading, glycosylation, deprotection, and cleavage can be performed using this setup.
一种标准的 HPLC 被应用于聚合物支撑的寡糖合成。基于溶液的试剂通过软件控制的溶剂输送系统输送。使用标准的紫外检测器可以实时监测反应的进度和完成情况。寡糖组装的所有步骤，包括加载、糖基化、脱保护和切割，都可以使用这种设置来完成。
