Institute for Dental Sciences, Hebrew University, Hadassah Medical Center, P.O. Box 12065, Jerusalem 91120, Israel.
Arch Oral Biol. 2012 Oct;57(10):1327-34. doi: 10.1016/j.archoralbio.2012.04.019. Epub 2012 May 29.
Lipophilic polyphenols in fruit beverages can avidly bind to surfaces of microorganisms and to blood cells and to impart upon them enhanced oxidant scavenging abilities (OSA). However, since many of the polyphenols are actually not fully soluble in water, they are therefore not available to act as effective antioxidant agents. We hypothesized that whole saliva, proteins such as albumin and mucin, human red blood cells and platelets, may all increase the "solubility" and availability of lipophilic antioxidant polyphenols thus increasing the OSA of whole saliva.
The OSA of whole un-stimulated human saliva, obtained from healthy donors and of combinations among saliva, mucin, blood cells, fruit beverages and reagent polyphenols were quantified by chemiluminescence, DPPH radical and tetrazolium reduction assays. Kinetics of the clearance of polyphenols from saliva after holding in the mouth for 30s of an extract from beverages cinnamon was assayed by the Folin Ciocalteu's and the luminescence assays.
OSA of fruit beverages and of reagent polyphenols were markedly increased by whole saliva, mucin and by red blood cells. Polyphenols associated with a cinnamon extract were retained in the oral cavity for several hours as measured by luminescence and Folin reagent techniques.
A new approach to explain the additional role of saliva and salivary proteins and of blood cells as enhancers of OSA of lipophilic polyphenols is presented. This might have a significant importance to assess complex interactions among polyphenols from nutrients, salivary antioxidants, salivary proteins and blood cells extravasated from injure capillaries during infection and inflammation.
水果饮料中的亲脂性多酚可以强烈结合微生物和血细胞表面，并赋予它们增强的抗氧化能力（OSA）。然而，由于许多多酚实际上在水中不完全溶解，因此它们不能作为有效的抗氧化剂发挥作用。我们假设全唾液、白蛋白和粘蛋白等蛋白质、人红细胞和血小板都可以增加亲脂性抗氧化多酚的“溶解度”和可用性，从而提高全唾液的 OSA。
通过化学发光、DPPH 自由基和四唑还原测定法，定量测定来自健康供体的未刺激的全人唾液的 OSA，以及唾液、粘蛋白、血细胞、水果饮料和试剂多酚之间的组合。通过 Folin Ciocalteu 试剂和发光测定法，测定含肉桂提取物的唾液在口中保持 30 秒后多酚从唾液中清除的动力学。
全唾液、粘蛋白和红细胞显著增加了水果饮料和试剂多酚的 OSA。与肉桂提取物结合的多酚通过发光和 Folin 试剂技术在口腔中保留数小时。
提出了一种新方法来解释唾液和唾液蛋白以及血细胞作为亲脂性多酚 OSA 增强剂的额外作用。这对于评估营养物质、唾液抗氧化剂、唾液蛋白和在感染和炎症期间从损伤毛细血管渗出的血细胞中的多酚之间的复杂相互作用可能具有重要意义。
