Bordonaro Salvatore, Guastella Anna Maria, Criscione Andrea, Zuccaro Antonio, Marletta Donata
DISPA, Sezione di Scienze delle Produzioni Animali, Università degli studi di Catania, Via Valdisavoia 5, 95123 Catania, Italy.
ScientificWorldJournal. 2012;2012:648427. doi: 10.1100/2012/648427. Epub 2012 Apr 29.
The genetic variability of Pantesco and other two Sicilian autochthonous donkey breeds (Ragusano and Grigio Siciliano) was assessed using a set of 14 microsatellites. The main goals were to describe the current differentiation among the breeds and to provide genetic information useful to safeguard the Pantesco breed as well as to manage Ragusano and Grigio Siciliano. In the whole sample, that included 108 donkeys representative of the three populations, a total of 85 alleles were detected. The mean number of alleles was lower in Pantesco (3.7), than in Grigio Siciliano and Ragusano (4.4 and 5.9, resp.). The three breeds showed a quite low level of gene diversity (He) ranging from 0.471 in Pantesco to 0.589 in Grigio. The overall genetic differentiation index (Fst) was quite high; more than 10% of the diversity was found among breeds. Reynolds' (D(R)) genetic distances, correspondence, and population structure analysis reproduced the same picture, revealing that, (a) Pantesco breed is the most differentiated in the context of the Sicilian indigenous breeds, (b) within Ragusano breed, two well-defined subgroups were observed. This information is worth of further investigation in order to provide suitable data for conservation strategies.
使用一组14个微卫星评估了潘泰斯科驴以及其他两个西西里本土驴品种（拉古萨诺驴和西西里灰驴）的遗传变异性。主要目标是描述这些品种目前的差异，并提供有助于保护潘泰斯科驴品种以及管理拉古萨诺驴和西西里灰驴的遗传信息。在包含代表这三个种群的108头驴的整个样本中，总共检测到85个等位基因。潘泰斯科驴的平均等位基因数（3.7个）低于西西里灰驴和拉古萨诺驴（分别为4.4个和5.9个）。这三个品种显示出相当低水平的基因多样性（He），范围从潘泰斯科驴的0.471到西西里灰驴的0.589。总体遗传分化指数（Fst）相当高；超过10%的多样性存在于品种之间。雷诺兹（D(R)）遗传距离、对应分析和种群结构分析呈现出相同的情况，表明：（a）在西西里本土品种中，潘泰斯科驴品种差异最大；（b）在拉古萨诺驴品种内，观察到两个界限分明的亚群。为了为保护策略提供合适的数据，这些信息值得进一步研究。
