植物乳杆菌 B7 抑制幽门螺杆菌生长并减轻胃炎症。
Lactobacillus plantarum B7 inhibits Helicobacter pylori growth and attenuates gastric inflammation.
机构信息
Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
World J Gastroenterol. 2012 May 28;18(20):2472-80. doi: 10.3748/wjg.v18.i20.2472.
AIM
To determine the anti-Helicobacter property of Lactobacillus plantarum B7 (L. plantarum) B7 supernatants in vitro and the protective effects of L. plantarum B7 on serum tumor necrosis factor-alpha (TNF-α), gastric malondialdehyde (MDA) level, apoptosis, and histopathology in Helicobacter pylori (H. pylori)-induced gastric inflammation in rats.
METHODS
In vitro, the inhibition of H. pylori growth was examined using L. plantarum B7 supernatants at pH 4 and pH 7 and at the concentration of 1×, 5× and 10× on plates inoculated with H. pylori. The inhibitory effect of H. pylori was interpreted by the size of the inhibition zone. In vitro, male Sprague-Dawley rats were randomly divided into four groups including group 1 (control group), group 2 (H. pylori infected group), group 3 (H. pylori infected with L. plantarum B7 10(6) CFUs/mL treated group) and group 4 (H. pylori infected with L. plantarum B7 10(10) CFUs/mL treated group). One week after H. pylori inoculation, L. plantarum B7 10(6) CFUs/mL or 10(10) CFUs/mL were fed once daily to group 3 and group 4, respectively, for one week. Blood and gastric samples were collected at the end of the study.
RESULTS
In vitro, at intact pH 4, mean inhibitory zone diameters of 8.5 mm and 13 mm were noted at concentrations of 5× and 10× of L. plantarum B7 supernatant disks, respectively. At adjusted pH 7, L. plantarum B7 supernatants at concentrations of 5× and 10× yielded mean inhibitory zone diameters of 6.5 mm and 11 mm, respectively. In the in vitro study, in group 2, stomach histopathology revealed mild to moderate H. pylori colonization and inflammation. The level of gastric MDA and epithelial cell apoptosis were significantly increased compared with group 1. The serum TNF-α level was significant decreased in group 3 compared with group 2 (P < 0.05). In addition, L. plantarum B7 treatments resulted in a significant improvement in stomach pathology, and decreased gastric MDA level and apoptotic epithelial cells.
CONCLUSION
L. plantarum B7 supernatant inhibits H. pylori growth. This inhibition was dose-dependent and greater at pH 4. Moreover, L. plantarum B7 attenuated H. pylori-induced gastric inflammation.
目的
确定植物乳杆菌 B7（L. plantarum）B7 上清液在体外的抗幽门螺杆菌特性，以及 L. plantarum B7 对幽门螺杆菌（H. pylori）诱导的大鼠胃炎症血清肿瘤坏死因子-α（TNF-α）、胃丙二醛（MDA）水平、细胞凋亡和组织病理学的保护作用。
方法
在体外，使用 pH 值为 4 和 7 的 L. plantarum B7 上清液以及浓度为 1×、5×和 10×的 L. plantarum B7 上清液，在接种有 H. pylori 的平板上检查 H. pylori 的生长抑制情况。通过抑菌圈的大小来解释 H. pylori 的抑制作用。在体外，雄性 Sprague-Dawley 大鼠随机分为四组，包括第 1 组（对照组）、第 2 组（H. pylori 感染组）、第 3 组（H. pylori 感染+L. plantarum B7 10（6）CFUs/mL 处理组）和第 4 组（H. pylori 感染+L. plantarum B7 10（10）CFUs/mL 处理组）。在 H. pylori 接种 1 周后，分别向第 3 组和第 4 组每日喂食 L. plantarum B7 10（6）CFUs/mL 或 10（10）CFUs/mL，持续 1 周。在研究结束时收集血液和胃样本。
结果
在体外，在完整 pH 值为 4 时，L. plantarum B7 上清液圆盘浓度为 5×和 10×时，平均抑菌圈直径分别为 8.5mm 和 13mm。在调整 pH 值为 7 时，L. plantarum B7 上清液在浓度为 5×和 10×时，平均抑菌圈直径分别为 6.5mm 和 11mm。在体外研究中，第 2 组胃组织病理学显示轻度至中度 H. pylori 定植和炎症。与第 1 组相比，胃 MDA 水平和上皮细胞凋亡显著增加。与第 2 组相比，第 3 组血清 TNF-α水平显著降低（P<0.05）。此外，L. plantarum B7 处理导致胃病理明显改善，胃 MDA 水平和凋亡上皮细胞减少。
结论
L. plantarum B7 上清液抑制 H. pylori 生长。这种抑制作用呈剂量依赖性，在 pH 值为 4 时更强。此外，L. plantarum B7 减轻了 H. pylori 诱导的胃炎症。
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