UNC Project, Lilongwe, Malawi.
J Clin Virol. 2012 Aug;54(4):318-20. doi: 10.1016/j.jcv.2012.05.003. Epub 2012 Jun 2.
In Sub-Saharan Africa, prevalence estimates of hepatitis C virus (HCV) vary widely.
To assess the prevalence of HCV infection among HIV-infected, pregnant women screened for a large clinical trial in Lilongwe, Malawi.
Plasma from 2041 HIV-infected, pregnant women was screened for anti-HCV IgG using a chemiluminiscent immunometric assay (CIA). Specimens with a signal-cut-off ratio≥1.00 were considered reactive and those with S/Co ratio<1.00 non-reactive. All CIA-reactive specimens were tested by a recombinant immunoblot assay (RIBA) for anti-HCV and by PCR for HCV RNA.
Of 2041 specimens, 110 (5.3%, 95% CI: 4.5-6.5%) were CIA reactive. Of the 109 CIA reactive specimens available for RIBA testing, 2 (1.8%) were positive, 28 (25.7%) were indeterminate, and 79 (72.5%) were negative. All CIA-reactive specimens were HCV RNA negative (n=110). The estimated HCV prevalence based on the screening assay alone was 5.3%; based on supplemental RIBA testing, the status of HCV infection remained indeterminate in 1.4% (28/2040, 95% CI: 0.1-2.0) and the prevalence of confirmed HCV infections was 0.1% (2/2040, 95% CI: 0-0.4%).
HCV seroprevalence among HIV-infected, pregnant women in Malawi confirmed by supplemental RIBA HCV 3.0 is low (0.1%); CIA showed a high false-reactivity rate in this population.
在撒哈拉以南非洲，丙型肝炎病毒（HCV）的流行率估计差异很大。
评估马拉维利隆圭一项大型临床试验中筛查的 HIV 感染孕妇中 HCV 感染的流行率。
使用化学发光免疫测定法（CIA）检测 2041 名 HIV 感染孕妇的血浆中抗 HCV IgG。将信号-临界比值≥1.00 的标本视为反应性，而 S/Co 比值<1.00 的标本视为非反应性。所有 CIA 反应性标本均用重组免疫印迹分析（RIBA）检测抗 HCV，并通过 PCR 检测 HCV RNA。
在 2041 份标本中，110 份（5.3%，95%CI：4.5-6.5%）为 CIA 反应性。在可用于 RIBA 检测的 109 份 CIA 反应性标本中，有 2 份（1.8%）为阳性，28 份（25.7%）为不确定，79 份（72.5%）为阴性。所有 CIA 反应性标本的 HCV RNA 均为阴性（n=110）。仅根据筛查检测，HCV 流行率估计为 5.3%；根据补充的 RIBA 检测，HCV 感染的状态在 1.4%（28/2040，95%CI：0.1-2.0）的患者中仍然不确定，而确诊的 HCV 感染率为 0.1%（2/2040，95%CI：0-0.4%）。
马拉维 HIV 感染孕妇经补充 RIBA HCV 3.0 确证的 HCV 血清流行率较低（0.1%）；CIA 在该人群中显示出高的假反应性率。
