Osorio Raquel, Yamauti Monica, Osorio Estrella, Román Julio S, Toledano Manuel
Department of Dental Materials, School of Dentistry, University of Granada, Granada Institute of Polymers, CSIC, Madrid, Spain.
Eur J Oral Sci. 2011 Oct;119(5):401-10. doi: 10.1111/j.1600-0722.2011.00853.x.
The aim of the study was to ascertain whether the addition of zinc to adhesives may decrease metalloproteinase-mediated collagen degradation without affecting bonding efficacy. Human dentin beams were treated with phosphoric acid, with Clearfil SE Bond Primer or with Clearfil SE Bond Primer plus ZnCl(2) (2 wt%). Acid-etched dentin was infiltrated with Single Bond, Single Bond plus ZnCl(2) (2 wt%), or Single Bond plus ZnO nanoparticles (10 wt%), and Clearfil SE Bond-primed dentin was infiltrated with Clearfil SE Bonding resin, Clearfil SE-Bonding resin with ZnCl(2) (2 wt%), or Clearfil SE-Bonding resin with ZnO nanoparticles (10 wt%). The C-terminal telopeptide concentrations were determined 24 h, and 1 and 4 wk after treatment. Microtensile bond strength to dentin was determined for the tested adhesives. Matrix metalloproteinases-mediated collagen degradation occurred in acid-etched and SE-primed dentin. Resin infiltration decreased collagen degradation. Lower collagen degradation was found for SE Bond than for Single Bond. Zinc-doped Single Bond resin always reduced collagen degradation, the ZnO particles being more effective than ZnCl(2) . Zinc-doped SE Bond reduced the liberation of C-terminal telopeptide only at 24 h. Bond strength to dentin was not decreased when Zn-doped resins were employed, except when ZnCl(2) was added to SE Primer. Zinc-doped resin reduced collagen degradation in Single Bond hybrid layers, but did not affect bond strength. The addition of zinc to SE Bond had no beneficial effects.
本研究的目的是确定在粘合剂中添加锌是否可以减少金属蛋白酶介导的胶原蛋白降解，同时不影响粘结效果。用人牙本质梁分别用磷酸、Clearfil SE Bond底漆或Clearfil SE Bond底漆加ZnCl₂（2 wt%）处理。酸蚀牙本质用Single Bond、Single Bond加ZnCl₂（2 wt%）或Single Bond加ZnO纳米颗粒（10 wt%）浸润，Clearfil SE Bond底漆处理过的牙本质用Clearfil SE Bonding树脂、含ZnCl₂（2 wt%）的Clearfil SE Bonding树脂或含ZnO纳米颗粒（10 wt%）的Clearfil SE Bonding树脂浸润。在处理后24小时、1周和4周测定C末端肽浓度。测定受试粘合剂与牙本质的微拉伸粘结强度。基质金属蛋白酶介导的胶原蛋白降解发生在酸蚀和SE底漆处理的牙本质中。树脂浸润减少了胶原蛋白降解。发现SE Bond的胶原蛋白降解低于Single Bond。掺杂锌的Single Bond树脂总是能减少胶原蛋白降解，ZnO颗粒比ZnCl₂更有效。掺杂锌的SE Bond仅在24小时时减少了C末端肽的释放。使用掺杂锌的树脂时，与牙本质的粘结强度没有降低，除非在SE底漆中添加了ZnCl₂。掺杂锌的树脂减少了Single Bond混合层中的胶原蛋白降解，但不影响粘结强度。在SE Bond中添加锌没有有益效果。
