Department of Obstetrics and Gynecology, The Affiliated Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
Int J Gynecol Cancer. 2012 Jul;22(6):1075-80. doi: 10.1097/IGC.0b013e3182574439.
Several placental microRNAs (miRNAs) have been identified as placenta-associated miRNAs with the potential of estimating the condition of the placenta. However, our understanding of these miRNAs is limited. The aim of this study was to determine the expression of 8 placenta-associated miRNAs (miR-512-3p, miR-517a, miR-517b, miR-518b, miR-519a, miR-1185, miR-1283, and miR-1323) in complete hydatidiform mole (CHM).
Samples were obtained from patients with CHM (CHM group, n = 12) and elective terminations of normal pregnancy (control group, n = 20). We detected differentially expressed placenta-associated miRNAs in placenta by quantitative real-time reverse transcriptase-polymerase chain reaction analysis. Subsequently, we assessed the expression location of differentially expressed miRNAs by in situ hybridization analysis.
Four placenta-associated miRNAs (miR-517a, miR-517b, miR-518b, and miR-519a) were underexpressed in the CHM group, compared with the control group (P < 0.01). When further investigating these 4 miRNAs with regard to in vivo localization by in situ hybridization, we found that 2 miRNAs (miR-517b and miR-518b) were detected exclusively in the trophoblast layer, with little signal (if any) observed in villous stroma cells.
The results show that 4 miRNAs (miR-517a, miR-517b, miR-518b, and miR-519a) are deregulated in CHM, which suggests the involvement of these miRNAs in the functions of CHM placenta.
已经鉴定出几种胎盘 microRNA（miRNA）为胎盘相关 miRNA，它们具有评估胎盘状况的潜力。然而，我们对这些 miRNA 的了解有限。本研究旨在确定 8 种胎盘相关 miRNA（miR-512-3p、miR-517a、miR-517b、miR-518b、miR-519a、miR-1185、miR-1283 和 miR-1323）在完全性葡萄胎（CHM）中的表达。
从 CHM 患者（CHM 组，n=12）和选择性终止正常妊娠（对照组，n=20）中获取样本。我们通过定量实时逆转录-聚合酶链反应分析检测胎盘差异表达的胎盘相关 miRNA。随后，通过原位杂交分析评估差异表达 miRNA 的表达位置。
与对照组相比，CHM 组有 4 种胎盘相关 miRNA（miR-517a、miR-517b、miR-518b 和 miR-519a）表达下调（P<0.01）。通过原位杂交进一步研究这 4 种 miRNA 的体内定位时，我们发现其中 2 种 miRNA（miR-517b 和 miR-518b）仅在滋养层中检测到，在绒毛间质细胞中几乎没有信号（如果有的话）。
结果表明，CHM 中存在 4 种 miRNA（miR-517a、miR-517b、miR-518b 和 miR-519a）失调，提示这些 miRNA 参与了 CHM 胎盘的功能。
