Department of Animal Science, Faculty of Science and Technology, Aarhus University, 8830 Tjele, Denmark.
Br J Nutr. 2012 Jun;107(11):1603-15. doi: 10.1017/S0007114511004909.
The objective of the present explorative study was to determine the absorption dynamics when feeding diets varying in types and levels of dietary fibre in a catheterised animal model. A total of six sows were fed a diet low in fibre (LF), a diet high in soluble fibre and a diet high in insoluble fibre in a repeated 3 × 3 cross-over design. Plasma samples were collected from the mesenteric artery and the portal vein to determine different absorption phases by ¹H NMR spectroscopy-based metabonomics. Time profiles were determined for plasma levels of specific metabolites and for the absorption of these metabolites from the small intestine. The LF diet resulted in a higher betaine concentration in the blood than the two high-fibre diets (P=0·008). This leads to higher plasma concentrations of methionine (P=0·0028) and creatine (P=0·020) of endogenous origin. In conclusion, the use of NMR spectroscopy for measuring nutrient uptake in the present study elucidated the relationship between betaine uptake and elevated creatine plasma concentrations.
本探索性研究的目的是在导管动物模型中确定不同类型和水平膳食纤维饮食的吸收动力学。共 6 头母猪采用低纤维饮食（LF）、高可溶性纤维饮食和高不溶性纤维饮食进行 3×3 交叉重复设计。通过基于 1H NMR 光谱的代谢组学，从肠系膜动脉和门静脉采集血浆样本，以确定不同的吸收阶段。确定了特定代谢物的血浆水平及其从小肠吸收的时间曲线。LF 饮食导致血液中甜菜碱浓度高于两种高纤维饮食（P=0·008）。这导致内源性蛋氨酸（P=0·0028）和肌酸（P=0·020）的血浆浓度更高。总之，本研究中使用 NMR 光谱测量营养物摄取阐明了甜菜碱摄取与升高的肌酸血浆浓度之间的关系。
