Edwin L. Steele Laboratory for Tumour Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
Integr Biol (Camb). 2012 Aug;4(8):857-62. doi: 10.1039/c2ib20061a. Epub 2012 Jun 6.
Here we describe a microfluidic device that accurately reproduces the dynamics of vascular anastomosis, the process by which vascular sprouts connect to achieve perfusion during angiogenesis. The micro-device features two parallel endothelial cell-lined vessel analogues separated by a 300 μm wide collagenous matrix into which the vessels can sprout and form perfused bridging connections. By accurately recapitulating anastomosis in vitro, the device will enable a new generation of studies of the mechanisms of angiogenesis and provide a novel and practical platform for drug screening.
在这里，我们描述了一种微流控装置，该装置能够准确再现血管吻合的动力学过程，即在血管生成过程中，血管芽通过连接实现灌注的过程。该微器件的特点是两个平行的内皮细胞衬里的血管模拟物，它们之间由 300μm 宽的胶原蛋白基质隔开，血管可以在其中发芽并形成灌注桥接连接。通过在体外准确再现吻合术，该装置将能够进行新一代的血管生成机制研究，并为药物筛选提供一个新颖实用的平台。
