Geisen R, Glenn E, Leistner L
Institute for Microbiology, Toxicology and Histology, Federal Centre of Meat Research, Kulmbach, Federal Republic of Germany.
Appl Environ Microbiol. 1990 Nov;56(11):3587-90. doi: 10.1128/aem.56.11.3587-3590.1990.
Penicillium camembertii was mutated and screened for cyclopiazonic acid-negative mutants. With a simple and rapid mini-extraction method for detection of cyclopiazonic acid production, we were able to isolate two strains which were affected in the production of this metabolite. One strain had completely lost the ability to synthesize detectable amounts of this secondary metabolite, whereas the other mutant produced 50 to 100 times less cyclopiazonic acid than the wild type. Also, the former strain had a changed morphology compared with the wild type. This morphological alteration appears to be coupled to the inability to produce cyclopiazonic acid because morphological revertants were able to synthesize cyclopiazonic acid to a level comparable to the wild type. The second mutant accumulated a new metabolite which was detectable by two-dimensional thin-layer chromatography. This new metabolite, however, appears not to be a direct precursor of cyclopiazonic acid.
对卡门柏青霉进行诱变并筛选环匹阿尼酸阴性突变体。通过一种简单快速的微量提取方法来检测环匹阿尼酸的产生，我们能够分离出两株在这种代谢物产生方面受到影响的菌株。一株完全丧失了合成可检测量这种次生代谢物的能力，而另一株突变体产生的环匹阿尼酸比野生型少50至100倍。此外，与野生型相比，前一株菌株的形态发生了变化。这种形态改变似乎与无法产生环匹阿尼酸有关，因为形态回复突变体能够合成与野生型相当水平的环匹阿尼酸。第二个突变体积累了一种新的代谢物，可通过二维薄层色谱法检测到。然而，这种新的代谢物似乎不是环匹阿尼酸的直接前体。
