North-West State Medical University n.a. I.I. Mechnikov, Piskarevskiy prospect 47-24, 195067 St. Petersburg, Russian Federation.
Benef Microbes. 2012 Jun 1;3(2):157-61. doi: 10.3920/BM2011.0023.
This article presents the results of original research conducted in St. Petersburg, Russia, which showed that the Helicobacter pylori strains isolated from patients with duodenal ulcer and chronic gastroduodenitis possess significantly higher levels of the pathogenicity islands (cag-PAI) genes than the strains isolated from healthy volunteers. The most frequently detected cag-PAI genes were cagA and cagH in the strains isolated from patients with chronic gastroduodenitis, and cagA and cagE in the strains isolated from patients with duodenal ulcer. A comparison of the clinical strains isolated from patients from St. Petersburg, Russia and patients from Dushanbe, Tajikistan showed that cagA gene was more prevalent in the strains from St. Petersburg. These findings demonstrate the necessity of implementation of molecular genetic identification of H. pylori in the clinical diagnostics practice reflecting the virulent genes profile of the strain. Addition of probiotics to the standard eradication therapy of H. pylori significantly improves the results of this therapy.
本文呈现了在俄罗斯圣彼得堡进行的原创研究结果，该研究表明，从十二指肠溃疡和慢性胃炎患者中分离出的幽门螺杆菌菌株具有明显更高水平的致病性岛（cag-PAI）基因，比从健康志愿者中分离出的菌株更高。在从慢性胃炎患者中分离出的菌株中，最常检测到的 cag-PAI 基因是 cagA 和 cagH，而在从十二指肠溃疡患者中分离出的菌株中，最常检测到的是 cagA 和 cagE。对来自俄罗斯圣彼得堡和塔吉克斯坦杜尚别的患者的临床分离株进行比较表明，cagA 基因在来自圣彼得堡的菌株中更为普遍。这些发现表明，在临床诊断实践中实施幽门螺杆菌的分子遗传鉴定是必要的，这反映了菌株的毒力基因特征。在幽门螺杆菌的标准根除治疗中添加益生菌可显著改善该治疗的结果。
