Jiang Lexia, Huang Baosheng, Tang Jiaming, Jiang Peihong, Chen Dongjie, Zhang Changfeng
Shandong Key Laboratory of Storage and Transportation Technology of Agricultural Products, Shandong Institute of Commerce and Technology, Jinan 250103, China.
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Foods. 2023 Jul 25;12(15):2820. doi: 10.3390/foods12152820.
Eugenol is a widely used fishery anesthetic. This study investigated the effects of various concentrations of eugenol on blood physiological and biochemical indexes, and muscle flavor, in crucian carp (). To determine the appropriate concentration of eugenol anesthetic for use in crucian carp transportation and production operations, we evaluated seven anesthesia groups of 20, 30, 40, 50, 60, 70, and 80 mg/L and one control group (without eugenol) to determine the effects on blood physiological and biochemical indexes, and muscle flavor. The red blood cells and platelets of crucian carp decreased significantly ( < 0.05) with eugenol treatment. With increasing eugenol concentration, the white blood cells and hemoglobin did not change significantly, whereas lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase increased significantly ( < 0.05). The content of phosphorus, magnesium, and sodium increased after anesthesia, whereas the content of potassium decreased with increasing eugenol concentration. After anesthesia, the content of albumin and total protein in the serum decreased with increasing eugenol concentration ( < 0.05); triglyceride first increased and subsequently decreased ( < 0.05); blood glucose content first increased and then decreased ( < 0.05); and no significant difference was observed in total cholesterol content ( > 0.05). No significant difference was observed in muscle glycogen and liver glycogen content after eugenol anesthesia ( > 0.05). The eugenol-based anesthesia test did not indicate major liver histomorphological effects, but the very small number of gill sheet edema cases observed requires further study. Analysis of electronic nose data indicated that eugenol treatment affected the flavor of the fish. The anesthesia concentration of 20-80 mg/L had some effect on the physiology and biochemistry of crucian carp, thus providing a reference for the application of eugenol in crucian carp transportation and experimental research.
丁香酚是一种广泛使用的渔业麻醉剂。本研究调查了不同浓度的丁香酚对鲫鱼血液生理生化指标及肌肉风味的影响。为确定用于鲫鱼运输和生产操作的丁香酚麻醉剂的适宜浓度，我们评估了20、30、40、50、60、70和80 mg/L的七个麻醉组以及一个对照组（无丁香酚），以确定其对血液生理生化指标及肌肉风味的影响。经丁香酚处理后，鲫鱼的红细胞和血小板显著减少（P<0.05）。随着丁香酚浓度的增加，白细胞和血红蛋白无显著变化，而乳酸脱氢酶、碱性磷酸酶、谷丙转氨酶和谷草转氨酶显著增加（P<0.05）。麻醉后磷、镁和钠的含量增加，而钾的含量随丁香酚浓度的增加而降低。麻醉后，血清中白蛋白和总蛋白的含量随丁香酚浓度的增加而降低（P<0.05）；甘油三酯先升高后降低（P<0.05）；血糖含量先升高后降低（P<0.05）；总胆固醇含量无显著差异（P>0.05）。丁香酚麻醉后肌肉糖原和肝糖原含量无显著差异（P>0.05）。基于丁香酚的麻醉试验未显示出主要的肝脏组织形态学影响，但观察到的极少数鳃片水肿病例需要进一步研究。电子鼻数据分析表明，丁香酚处理会影响鱼的风味。20 - 80 mg/L的麻醉浓度对鲫鱼的生理生化有一定影响，从而为丁香酚在鲫鱼运输和实验研究中的应用提供了参考。
