Palmer Gregory M, Fontanella Andrew N, Shan Siqing, Dewhirst Mark W
Department of Radiation Oncology, Duke University, Durham, NC, USA.
Methods Mol Biol. 2012;872:31-50. doi: 10.1007/978-1-61779-797-2_3.
Fluorescent proteins enable in vivo characterization of a wide and growing array of morphological and functional biomarkers. To fully capitalize on the spatial and temporal information afforded by these reporter proteins, a method for imaging these proteins at high resolution longitudinally is required. This chapter describes the use of window chamber models as a means of imaging fluorescent proteins and other optical parameters. Such models essentially involve surgically implanting a window through which tumor or normal tissue can be imaged using existing microscopy techniques. This enables acquisition of high-quality images down to the cellular or subcellular scale, exploiting the diverse array of optical contrast mechanisms, while also maintaining the native microenvironment of the tissue of interest. This makes these techniques applicable to a wide array of problems in the biomedical sciences.
荧光蛋白能够在体内对大量且不断增加的形态和功能生物标志物进行表征。为了充分利用这些报告蛋白提供的空间和时间信息，需要一种纵向高分辨率成像这些蛋白的方法。本章描述了使用窗口室模型作为对荧光蛋白和其他光学参数进行成像的手段。此类模型本质上涉及通过手术植入一个窗口，利用现有的显微镜技术可以透过该窗口对肿瘤或正常组织进行成像。这能够利用多种光学对比机制获取低至细胞或亚细胞尺度的高质量图像，同时还能维持感兴趣组织的天然微环境。这使得这些技术适用于生物医学科学中的一系列广泛问题。
