用于骨折治疗的不锈钢植入物上的抗菌颗粒银涂层

Antimicrobial Particulate Silver Coatings on Stainless Steel Implants for Fracture Management.

作者信息

Devasconcellos Paul, Bose Susmita, Beyenal Haluk, Bandyopadhyay Amit, Zirkle Lewis G

机构信息

W.M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA USA.

出版信息

Mater Sci Eng C Mater Biol Appl. 2012 Jul 1;32(5):1112-1120. doi: 10.1016/j.msec.2012.02.020.

We have used particulate silver coating on stainless steel to prevent in vivo bacterial infection. Stainless steel is commonly used as an implant material for fracture management. The antimicrobial use of silver has been well documented and studied, therefore the novelty of this research is the use of a particulate coating as well as facing the real world challenges of a fracture repair implant. The variable parameters for applying the coating were time of deposition, silver solution concentration, voltage applied, heat treatment temperature between 400 to 500 °C and time. The resultant coating is shown to be non-toxic to human osteoblasts using an MTT assay for proliferation and SEM images for morphology. In vitro silver release studies of various treatments were done using simulated body fluid. The bactericidal effects were tested by challenging the coatings with P. aeruginosa in a bioreactor and compared against uncoated stainless steel. A 13-fold reduction in bacteria was observed at 24 hours and proved to be statistically significant.

我们已在不锈钢上使用颗粒银涂层来预防体内细菌感染。不锈钢常用于骨折治疗的植入材料。银的抗菌用途已有充分记录和研究，因此本研究的新颖之处在于使用颗粒涂层以及面对骨折修复植入物在现实世界中的挑战。施加涂层的可变参数包括沉积时间、银溶液浓度、施加的电压、400至500°C之间的热处理温度以及时间。使用MTT法进行增殖检测和SEM图像观察形态，结果表明所得涂层对人成骨细胞无毒。使用模拟体液对各种处理进行体外银释放研究。通过在生物反应器中用铜绿假单胞菌挑战涂层并与未涂层的不锈钢进行比较来测试杀菌效果。在24小时时观察到细菌数量减少了13倍，且经统计学证明具有显著性。