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激光辅助磷酸三钙涂层的体外抗菌及生物学特性

In vitro antimicrobial and biological properties of laser assisted tricalcium phosphate coating.

作者信息

Roy Mangal, Bandyopadhyay Amit, Bose Susmita

机构信息

W.M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.

出版信息

Mater Sci Eng C Mater Biol Appl. 2009 Aug 1;29(6):1965-1968. doi: 10.1016/j.msec.2009.03.009.

Abstract

Implant related infections are of great concern in modern surgery. In order to improve the implant performance and to reduce implant related infections, titanium (Ti) surface was modified to simultaneously improve cell- materials interactions and antimicrobial activity. Ti surface was first coated with tricalcium phosphate (TCP) using Laser Engineered Net Shaping (LENS™) to improve biocompatibility. Silver (Ag) was then electrodeposited from different concentrations of silver nitrate (AgNO(3)) solutions to improve the antimicrobial activity. The Ag-TCP coatings were tested for cytotoxicy with human osteoblast cells. The antimicrobial activities of the Ag-TCP coatings were evaluated using Pseudomonas aeruginosa and Staphylococcus aureus bacteria. In vitro bacterial adhesion study indicated a significant reduction in bacterial colony on Ag-TCP coated surfaces when compared to TCP coated surface.

摘要

植入物相关感染是现代外科手术中备受关注的问题。为了提高植入物性能并减少植入物相关感染,对钛(Ti)表面进行了改性,以同时改善细胞与材料的相互作用以及抗菌活性。首先使用激光工程化净成形(LENS™)技术在Ti表面涂覆磷酸三钙(TCP)以提高生物相容性。然后从不同浓度的硝酸银(AgNO₃)溶液中电沉积银(Ag)以提高抗菌活性。对Ag-TCP涂层进行了人成骨细胞的细胞毒性测试。使用铜绿假单胞菌和金黄色葡萄球菌评估了Ag-TCP涂层的抗菌活性。体外细菌粘附研究表明,与TCP涂层表面相比,Ag-TCP涂层表面的细菌菌落显著减少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebbb/3065785/515340bdf090/nihms-164502-f0001.jpg

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