随机对照试验中身体活动对脂肪组织功能的影响。
Functional changes in adipose tissue in a randomised controlled trial of physical activity.
机构信息
Atherosclerosis Research Unit,, Department of Medicine, (Solna) Karolinska Institutet, Stockholm, Sweden.
出版信息
Lipids Health Dis. 2012 Jun 21;11:80. doi: 10.1186/1476-511X-11-80.
BACKGROUND
A sedentary lifestyle predisposes to cardiometabolic diseases. Lifestyle changes such as increased physical activity improve a range of cardiometabolic risk factors. The objective of this study was to examine whether functional changes in adipose tissue were related to these improvements.
METHODS
Seventy-three sedentary, overweight (mean BMI 29.9 ± 3.2 kg/m2) and abdominally obese, but otherwise healthy men and women (67.6 ± 0.5 years) from a randomised controlled trial of physical activity on prescription over a 6-month period were included (control n = 43, intervention n = 30). Detailed examinations were carried out at baseline and at follow-up, including fasting blood samples, a comprehensive questionnaire and subcutaneous adipose tissue biopsies for fatty acid composition analysis (n = 73) and quantification of mRNA expression levels of 13 candidate genes (n = 51), including adiponectin, leptin and inflammatory cytokines.
RESULTS
At follow-up, the intervention group had a greater increase in exercise time (+137 min/week) and a greater decrease in body fat mass (-1.5 kg) compared to the control subjects (changes of 0 min/week and -0.5 kg respectively). Circulating concentrations of adiponectin were unchanged, but those of leptin decreased significantly more in the intervention group (-1.8 vs -1.1 ng/mL for intervention vs control, P < 0.05). The w6-polyunsaturated fatty acid content, in particular linoleic acid (18:2w6), of adipose tissue increased significantly more in the intervention group, but the magnitude of the change was small (+0.17 vs +0.02 percentage points for intervention vs control, P < 0.05). Surprisingly leptin mRNA levels in adipose tissue increased in the intervention group (+107% intervention vs -20% control, P < 0.05), but changes in expression of the remaining genes did not differ between the groups.
CONCLUSIONS
After a 6-month period of increased physical activity in overweight elderly individuals, circulating leptin concentrations decreased despite increased levels of leptin mRNA in adipose tissue. Otherwise, only minor changes occurred in adipose tissue, although several improvements in metabolic parameters accompanied the modest increase in physical activity.
背景
久坐的生活方式易导致代谢相关心血管疾病。生活方式的改变，如增加身体活动，可改善多种代谢相关心血管疾病的风险因素。本研究旨在探讨脂肪组织的功能变化是否与这些改善相关。
方法
本研究纳入了一项为期 6 个月的运动处方对照随机临床试验中的 73 名久坐、超重（平均 BMI 29.9±3.2kg/m2）和腹型肥胖但其他方面健康的男性和女性（67.6±0.5 岁）（对照组 n=43，干预组 n=30）。在基线和随访时进行了详细检查，包括空腹血样、综合问卷和皮下脂肪组织活检，以分析脂肪酸组成（n=73）和 13 个候选基因（n=51）的 mRNA 表达水平，包括脂联素、瘦素和炎性细胞因子。
结果
随访时，与对照组相比，干预组的运动时间增加了 137 分钟/周（+137 min/week），体脂量减少了 1.5kg（-1.5 kg）。循环中脂联素浓度无变化，但瘦素浓度下降更为显著（干预组-1.8ng/mL，对照组-1.1ng/mL，P<0.05）。脂肪组织中的 w6-多不饱和脂肪酸含量，特别是亚油酸（18:2w6），在干预组中显著增加，但变化幅度较小（+0.17 个百分点比+0.02 个百分点，P<0.05）。令人惊讶的是，干预组脂肪组织中的瘦素 mRNA 水平增加（+107%干预组比-20%对照组，P<0.05），但其余基因的表达变化在两组之间没有差异。
结论
在超重老年人中进行 6 个月的身体活动增加后，尽管脂肪组织中的瘦素 mRNA 水平增加，但循环中的瘦素浓度下降。此外，尽管身体活动适度增加伴随多种代谢参数改善，但脂肪组织仅发生了较小的变化。
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