硼酸探针作为实时监测过氧亚硝酸盐和过氧化物的诊断工具。
Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides.
机构信息
Department of Biophysics, Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
出版信息
Chem Res Toxicol. 2012 Sep 17;25(9):1793-9. doi: 10.1021/tx300164j. Epub 2012 Jun 25.
Abstract
Boronates, a group of organic compounds, are emerging as one of the most effective probes for detecting and quantifying peroxynitrite, hypochlorous acid, and hydrogen peroxide. Boronates react with peroxynitrite nearly a million times faster than with hydrogen peroxide. Boronate-containing fluorogenic compounds have been used to monitor real time generation of peroxynitrite in cells and for imaging hydrogen peroxide in living animals. This perspective highlights potential applications of boronates and other fluorescent probes to high-throughput analyses of peroxynitrite and hydroperoxides in toxicological studies.
摘要
硼酸盐是一类有机化合物，它们作为检测和定量检测过氧亚硝酸盐、次氯酸和过氧化氢的最有效探针之一而崭露头角。硼酸盐与过氧亚硝酸盐的反应速度比与过氧化氢快近一百万倍。含硼酸盐的荧光化合物已被用于实时监测细胞中过氧亚硝酸盐的产生，并用于在活体动物中成像过氧化氢。本观点强调了硼酸盐和其他荧光探针在毒理学研究中对过氧亚硝酸盐和过氧化物进行高通量分析的潜在应用。
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