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评价生物场处理剂量和距离对癌细胞死亡的影响。

Evaluation of biofield treatment dose and distance in a model of cancer cell death.

机构信息

California Pacific Medical Center Research Institute, San Francisco, CA 94107, USA.

出版信息

J Altern Complement Med. 2013 Feb;19(2):124-7. doi: 10.1089/acm.2011.0950. Epub 2012 Jun 25.

Abstract

OBJECTIVE

This study assessed the potential influence of biofield treatment on cultured human cancer cells and whether such influence was affected by varying the duration of the treatment (dose) or the distance between the biofield practitioner and the target cells.

DESIGN

Biofield treatment dosage was assessed from a short distance (0.25 meters) in three independent experiments involving 1, 2, or 5 treatments, along with another set of three independent and comparable mock experiments. Biofield treatment distance was assessed at 0.25, 25, and ∼ 2000 meters involving two treatments in three independent experiments along with another set of three mock experiments.

INTERVENTION

Biofield treatments were delivered by a highly acclaimed biofield practitioner with the intention of diminishing growth of the cells or inducing cancer-cell death.

OUTCOME MEASURE

Cell viability was quantified 20 hours after treatments, using a spectrophotometric assay for live-cell counting. The dependent measure for each experiment was the log ratio of the cell viability values of treated samples (biofield or mock) over the values of untreated control samples.

RESULTS

A trend of decreasing cell viability with increasing biofield dose was evident in the first set of experiments assessing dose-response; however, no such effect was evident in the second set of experiments evaluating biofield treatment distance. Mock experiments yielded relatively stable viability ratios in both sets of experiments. Linear regression analysis and hypothesis testing of the data taken as a whole did not yield statistical significance at p<0.05.

CONCLUSIONS

These results represent the first indication of a biofield treatment dose-response in a controlled laboratory setting. The data are inconclusive because of the inability of reproduce the cellular response in a replicate experiment.

摘要

目的

本研究评估了生物场处理对培养的人类癌细胞的潜在影响,以及这种影响是否会因处理持续时间(剂量)或生物场治疗师与目标细胞之间的距离而变化。

设计

在三个独立的实验中,评估了从短距离(0.25 米)进行生物场处理的剂量,涉及 1、2 或 5 次处理,以及另外三组独立且可比的模拟实验。在三个独立的实验中,评估了生物场处理距离为 0.25、25 和 ∼2000 米,涉及两次处理,以及另外三组模拟实验。

干预

生物场治疗由一位备受赞誉的生物场治疗师提供,旨在减少细胞生长或诱导癌细胞死亡。

结果测量

用分光光度法检测活细胞计数来量化处理后 20 小时的细胞活力。每个实验的因变量是处理样本(生物场或模拟)的细胞活力值与未处理对照样本的对数比。

结果

在评估剂量反应的第一组实验中,细胞活力随生物场剂量增加而降低的趋势明显;然而,在评估生物场处理距离的第二组实验中,没有出现这种影响。在两组实验中,模拟实验都产生了相对稳定的活力比值。对整体数据进行线性回归分析和假设检验,未得出 p<0.05 的统计学意义。

结论

这些结果代表了在受控实验室环境中首次出现生物场处理剂量反应的迹象。由于无法在重复实验中再现细胞反应,数据尚无定论。

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