Northwestern University, Department of Biomedical Engineering, Evanston, Illinois 60208, USA.
J Biomed Opt. 2012 Jun;17(6):061206. doi: 10.1117/1.JBO.17.6.061206.
Photoacoustic ophthalmoscopy (PAOM) is a newly developed retinal imaging technology that holds promise for both fundamental investigation and clinical diagnosis of several blinding diseases. Hence, integrating PAOM with other existing ophthalmic imaging modalities is important to identify and verify the strengths of PAOM compared with the established technologies and to provide the foundation for more comprehensive multimodal imaging. To this end, we developed a retinal imaging platform integrating PAOM with scanning laser ophthalmoscopy (SLO), spectral-domain optical coherence tomography (SD-OCT), and fluorescein angiography (FA). In the system, all the imaging modalities shared the same optical scanning and delivery mechanisms, which enabled registered retinal imaging from all the modalities. High-resolution PAOM, SD-OCT, SLO, and FA images were acquired in both albino and pigmented rat eyes. The reported in vivo results demonstrate the capability of the integrated system to provide comprehensive anatomic imaging based on multiple optical contrasts.
光声眼底镜（PAOM）是一种新开发的视网膜成像技术，有望用于多种致盲性疾病的基础研究和临床诊断。因此，将 PAOM 与其他现有的眼科成像方式相结合，对于识别和验证 PAOM 与现有技术相比的优势，以及为更全面的多模态成像提供基础非常重要。为此，我们开发了一种视网膜成像平台，将光声眼底镜与扫频激光检眼镜（SLO）、谱域光学相干断层扫描（SD-OCT）和荧光素血管造影（FA）相结合。在该系统中，所有成像方式都共享相同的光学扫描和传输机制，从而实现了所有方式的注册视网膜成像。我们在白化和色素沉着大鼠眼中获得了高分辨率的光声眼底镜、SD-OCT、SLO 和 FA 图像。报告的体内结果表明，该集成系统能够基于多种光学对比提供全面的解剖成像。
