Wenzhou Medical College, School of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
J Biomed Opt. 2012 Jun;17(6):066013. doi: 10.1117/1.JBO.17.6.066013.
This study is designed to test the repeatability of the quantitative analysis of intraretinal layer thickness and cup-disc ratio of the optic nerve head using ultra-high resolution optical coherence tomography (UHR-OCT). Group A, containing 23 eyes of 12 healthy subjects, was imaged twice and group B, containing eight eyes of four subjects, was imaged three times. Intraretinal layers were segmented manually and the cup-to-disc ratio of the optic nerve head was analyzed. Custom-built automatic segmentation software was also used to segment a set of images for comparison. A total of nine intraretinal layers were visualized and extracted manually. With group A, the central foveal thickness was 186.4 ± 15.9 μm (mean ± SD). The average retinal thickness was 296.4 ± 21.3 μm. The best repeatability, obtained when two repeated scans were taken, was obtained for the outer nuclear layer followed by the ganglion cell layer, the inner nuclear layer, the retinal nerve fiber layer and the worst was obtained for the outer segment. The intraclass correlation ranged from 0.824 to 0.997. The coefficients of repeatability ranged from 3.24 to 18.3 μm, corresponding to 1.47% to 26.20%. With group B, high interclass correlations were found and the automatic segmentation results were compatible with the manual results. Our results indicated that more retinal features might be imageable using UHR-OCT.
本研究旨在测试使用超高分辨率光学相干断层扫描（UHR-OCT）对视网膜内各层厚度和视盘杯盘比的定量分析的可重复性。A 组包含 12 名健康受试者的 23 只眼，进行了两次成像，B 组包含 4 名受试者的 8 只眼，进行了三次成像。手动分割视网膜内各层，并分析视盘的杯盘比。还使用定制的自动分割软件对一组图像进行分割以进行比较。总共手动可视化和提取了 9 个视网膜内各层。A 组中，中央凹视网膜厚度为 186.4±15.9μm（平均值±标准差）。平均视网膜厚度为 296.4±21.3μm。两次重复扫描时获得的最佳可重复性依次为外核层、神经节细胞层、内核层、视网膜神经纤维层，最差的是外节。组内相关系数范围为 0.824 至 0.997。重复性系数范围为 3.24 至 18.3μm，对应于 1.47%至 26.20%。B 组中，发现组间相关性较高，自动分割结果与手动结果一致。我们的结果表明，UHR-OCT 可能可以显示更多的视网膜特征。
