一种用于在亚细胞分辨率下对活神经元细胞中不稳定的 Fe(3+)进行成像的turn-on 荧光传感器。
A turn-on fluorescent sensor for imaging labile Fe(3+) in live neuronal cells at subcellular resolution.
机构信息
Department of Chemistry and Biochemistry and UMass Cranberry Health Research Center, University of Massachusetts Dartmouth, 285 Old Westport Road, Dartmouth, MA 02747, USA.
出版信息
Chembiochem. 2012 Jul 23;13(11):1569-73. doi: 10.1002/cbic.201200202. Epub 2012 Jun 26.
An eye for an iron: A highly sensitive, selective and reversible turn-on Fe(3+) sensor for imaging labile Fe(3+) in live cells at subcellular resolution is reported. The sensor can respond to changes in intracellular Fe(3+) levels and was used to image endogenous chelatable Fe(3+) in live human neuroblastoma SH-SY5Y cells, with two Fe(3+) pools being identified in mitochondria and endosomes/ lysosomes for the first time.
以眼还眼
报道了一种高灵敏度、选择性和可逆的铁（III）传感器，可在亚细胞分辨率下对活细胞中不稳定的铁（III）进行成像。该传感器可以响应细胞内铁（III）水平的变化，并用于对活的人神经母细胞瘤 SH-SY5Y 细胞中的内源性可螯合铁（III）进行成像，首次在其中鉴定出线粒体和内体/溶酶体中的两个铁（III）池。
Zotero 插件