一种新型荧光“开启”化学传感器，用于从水溶液中纳米摩尔级检测 Fe(III)，并将其应用于活细胞成像。
A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging.
机构信息
School of Chemical Sciences, North Maharashtra University, P. B. No. 80, Jalgaon 425001, MS, India.
Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
出版信息
Biosens Bioelectron. 2014 Nov 15;61:612-7. doi: 10.1016/j.bios.2014.06.017. Epub 2014 Jun 14.
An electronically active and spectral sensitive fluorescent "turn-on" chemosensor (BTP-1) based on the benzo-thiazolo-pyrimidine unit was designed and synthesized for the highly selective and sensitive detection of Fe(3+) from aqueous medium. With Fe(3+), the sensor BTP-1 showed a remarkable fluorescence enhancement at 554 nm (λex = 314 nm) due to the inhibition of photo-induced electron transfer. The sensor formed a host-guest complex in 1:1 stoichiometry with the detection limit down to 0.74 nM. Further, the sensor was successfully utilized for the qualitative and quantitative intracellular detection of Fe(3+) in two liver cell lines i.e., HepG2 cells (human hepatocellular liver carcinoma cell line) and HL-7701 cells (human normal liver cell line) by a confocal imaging technique.
一种基于苯并噻唑并嘧啶单元的具有电子活性和光谱敏感性的荧光“开启”型化学传感器（BTP-1）被设计和合成，用于从水相中高度选择性和灵敏地检测 Fe(3+)。与 Fe(3+) 结合后，传感器 BTP-1 在 554nm（λex = 314nm）处显示出显著的荧光增强，这是由于光诱导电子转移的抑制。传感器以 1:1 的化学计量比形成主客体配合物，检测限低至 0.74nM。此外，该传感器还成功地通过共聚焦成像技术用于两种肝细胞系（即 HepG2 细胞（人肝癌细胞系）和 HL-7701 细胞（人正常肝细胞系））中 Fe(3+)的定性和定量细胞内检测。
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