The Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province, Harbin, 150090 Heilongjiang, People's Republic of China.
Mol Biol Rep. 2012 Oct;39(10):9435-43. doi: 10.1007/s11033-012-1808-4. Epub 2012 Jun 28.
100-Seed weight (100-SW) of soybean is an important but complicated quantitative trait to yield. This study was focus on the quantitative trait loci (QTLs) of soybean 100-SW from 2006 to 2010, using recombination inbred lines population that was derived from a cross between Charleston and Dongnong 594. A total of 23 QTLs for 100-SW were detected in the linkage group C2, D1a, F, G and O. Nine QTLs were identified by composite interval mapping including one QTL with the minimum confidence interval (CI) of 1.3 cM, while 14 QTLs by multiple interval mapping. Furthermore, 94 reported QTLs of 100-SW were integrated with our QTL mapping results using BioMercator. As a result, 15 consensus QTLs and their corresponding markers were identified. The minimum CI was reduced to 1.52 cM by the combination of meta-analysis. These findings may merit fine-mapping of these QTL in soybean.
大豆的百粒重(100-SW)是一个重要但复杂的产量数量性状。本研究聚焦于 2006 年至 2010 年期间,使用源自 Charleston 和 Dongnong 594 杂交的重组自交系群体,对大豆 100-SW 的数量性状基因座(QTLs)进行了研究。在连锁群 C2、D1a、F、G 和 O 中,共检测到 23 个 100-SW 的 QTL。通过复合区间作图鉴定了 9 个 QTL,其中一个 QTL 的最小置信区间(CI)为 1.3cM,而通过多区间作图鉴定了 14 个 QTL。此外,使用 BioMercator 将 94 个报告的 100-SW QTL 与我们的 QTL 作图结果进行了整合。结果,鉴定了 15 个共识 QTL 及其相应的标记。通过荟萃分析的组合,最小 CI 降低至 1.52cM。这些发现可能值得对这些 QTL 在大豆中的精细定位。