Saunter C D, Semprini S, Buckley C, Mullins J, Girkin J M
Biomed Opt Express. 2012 Jun 1;3(6):1274-8. doi: 10.1364/BOE.3.001274. Epub 2012 May 4.
In this paper we report the design, testing and use of a scannerless probe specifically for minimally invasive imaging of deep tissue in vivo with an epi-fluorescence modality. The probe images a 500 μm diameter field of view through a 710 μm outer diameter probe with a maximum tissue penetration depth of 15 mm specifically configured for eGFP imaging. Example results are given from imaging the pituitary gland of rats and zebrafish hearts with lateral resolution of 2.5 μm.
在本文中，我们报告了一种无扫描探头的设计、测试及应用，该探头专为采用落射荧光成像方式对体内深部组织进行微创成像而设计。该探头通过外径为710μm的探头对直径500μm的视场进行成像，其最大组织穿透深度为15mm，特别配置用于增强型绿色荧光蛋白（eGFP）成像。文中给出了对大鼠垂体和斑马鱼心脏成像的示例结果，横向分辨率为2.5μm。
