• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

红细胞内期疟原虫感染导致中性粒细胞麻痹。

Neutrophil paralysis in Plasmodium vivax malaria.

机构信息

Laboratório de Imunopatologia, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.

出版信息

PLoS Negl Trop Dis. 2012;6(6):e1710. doi: 10.1371/journal.pntd.0001710. Epub 2012 Jun 26.

DOI:10.1371/journal.pntd.0001710
PMID:22745844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3383745/
Abstract

BACKGROUND

The activation of innate immune responses by Plasmodium vivax results in activation of effector cells and an excessive production of pro-inflammatory cytokines that may culminate in deleterious effects. Here, we examined the activation and function of neutrophils during acute episodes of malaria.

MATERIALS AND METHODS

Blood samples were collected from P. vivax-infected patients at admission (day 0) and 30-45 days after treatment with chloroquine and primaquine. Expression of activation markers and cytokine levels produced by highly purified monocytes and neutrophils were measured by the Cytometric Bead Assay. Phagocytic activity, superoxide production, chemotaxis and the presence of G protein-coupled receptor (GRK2) were also evaluated in neutrophils from malaria patients.

PRINCIPAL FINDINGS

Both monocytes and neutrophils from P. vivax-infected patients were highly activated. While monocytes were found to be the main source of cytokines in response to TLR ligands, neutrophils showed enhanced phagocytic activity and superoxide production. Interestingly, neutrophils from the malaria patients expressed high levels of GRK2, low levels of CXCR2, and displayed impaired chemotaxis towards IL-8 (CXCL8).

CONCLUSION

Activated neutrophils from malaria patients are a poor source of pro-inflammatory cytokines and display reduced chemotactic activity, suggesting a possible mechanism for an enhanced susceptibility to secondary bacterial infection during malaria.

摘要

背景

间日疟原虫激活先天免疫反应会导致效应细胞的激活和促炎细胞因子的过度产生，这可能导致有害影响。在这里，我们研究了疟原虫感染患者中性粒细胞在急性疟疾病例中的激活和功能。

材料和方法

在接受氯喹和伯氨喹治疗 30-45 天后，采集间日疟原虫感染患者入院时（第 0 天）和入院时的血样。通过流式细胞术检测高度纯化的单核细胞和中性粒细胞产生的激活标志物和细胞因子水平。还评估了疟疾病例中性粒细胞的吞噬活性、超氧化物产生、趋化性和 G 蛋白偶联受体（GRK2）的存在。

主要发现

来自间日疟原虫感染患者的单核细胞和中性粒细胞均高度激活。虽然单核细胞被发现是对 TLR 配体产生细胞因子的主要来源，但中性粒细胞表现出增强的吞噬活性和超氧化物产生。有趣的是，疟疾病例中的中性粒细胞表达高水平的 GRK2、低水平的 CXCR2，并表现出对白细胞介素-8（CXCL8）的趋化性受损。

结论

疟疾病例中激活的中性粒细胞是促炎细胞因子的不良来源，并且表现出降低的趋化性活性，这表明在疟疾期间继发性细菌感染易感性增强的可能机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/20a5a29a728f/pntd.0001710.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/548f2a560f70/pntd.0001710.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/e4b4d0e4f189/pntd.0001710.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/6eb2cf48cbb1/pntd.0001710.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/987c46d098cd/pntd.0001710.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/85c8d94834d6/pntd.0001710.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/20a5a29a728f/pntd.0001710.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/548f2a560f70/pntd.0001710.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/e4b4d0e4f189/pntd.0001710.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/6eb2cf48cbb1/pntd.0001710.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/987c46d098cd/pntd.0001710.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/85c8d94834d6/pntd.0001710.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd84/3383745/20a5a29a728f/pntd.0001710.g006.jpg

相似文献

1
Neutrophil paralysis in Plasmodium vivax malaria.红细胞内期疟原虫感染导致中性粒细胞麻痹。
PLoS Negl Trop Dis. 2012;6(6):e1710. doi: 10.1371/journal.pntd.0001710. Epub 2012 Jun 26.
2
The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.在间日疟原虫急性疟疾期间，CD14+CD16+炎性单核细胞亚群表现出线粒体活性增加和效应功能增强。
PLoS Pathog. 2014 Sep 18;10(9):e1004393. doi: 10.1371/journal.ppat.1004393. eCollection 2014 Sep.
3
Analysis of the lymphocyte cell population during malaria caused by Plasmodium vivax and its correlation with parasitaemia and thrombocytopaenia.分析间日疟原虫引起的疟疾期间的淋巴细胞群及其与寄生虫血症和血小板减少症的相关性。
Malar J. 2018 Aug 20;17(1):303. doi: 10.1186/s12936-018-2443-x.
4
Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes.疟原虫感染改变人单核细胞中线粒体代谢。
mBio. 2021 Aug 31;12(4):e0124721. doi: 10.1128/mBio.01247-21. Epub 2021 Jul 27.
5
The robust and modulated biomarker network elicited by the Plasmodium vivax infection is mainly mediated by the IL-6/IL-10 axis and is associated with the parasite load.由间日疟原虫感染引起的稳健且调节良好的生物标志物网络主要由 IL-6/IL-10 轴介导，并与寄生虫载量相关。
J Immunol Res. 2014;2014:318250. doi: 10.1155/2014/318250. Epub 2014 Mar 18.
6
On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease.间日疟原虫疟疾期间的细胞因子/趋化因子网络：理解该疾病的新见解
Malar J. 2017 Jan 24;16(1):42. doi: 10.1186/s12936-017-1683-5.
7
Networking the host immune response in Plasmodium vivax malaria.网络连接间日疟原虫宿主免疫反应。
Malar J. 2013 Feb 21;12:69. doi: 10.1186/1475-2875-12-69.
8
Immune response pattern in recurrent Plasmodium vivax malaria.间日疟复发时的免疫反应模式
Malar J. 2016 Aug 31;15(1):445. doi: 10.1186/s12936-016-1501-5.
9
Malaria systems immunology: Plasmodium vivax induces tolerance during primary infection through dysregulation of neutrophils and dendritic cells.疟疾系统免疫学：间日疟原虫在初次感染期间通过调节中性粒细胞和树突状细胞诱导耐受。
J Infect. 2018 Nov;77(5):440-447. doi: 10.1016/j.jinf.2018.09.005. Epub 2018 Sep 22.
10
A preliminary study on pro- and anti-inflammatory cytokine profiles in Plasmodium vivax malaria patients from central zone of India.印度中部地区间日疟原虫疟疾患者促炎和抗炎细胞因子谱的初步研究。
Acta Trop. 2010 Mar;113(3):263-8. doi: 10.1016/j.actatropica.2009.11.009. Epub 2009 Dec 1.

引用本文的文献

1
Potential Benefits of Lycopene Consumption: Rationale for Using It as an Adjuvant Treatment for Malaria Patients and in Several Diseases.番茄红素的潜在益处：将其用作疟疾患者辅助治疗和几种疾病治疗手段的基本原理。
Nutrients. 2022 Dec 14;14(24):5303. doi: 10.3390/nu14245303.
2
Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots.GRK 抗体在 Western Blots 中用于 GRK 同工型的个体检测和定量的适用性。
Int J Mol Sci. 2022 Jan 21;23(3):1195. doi: 10.3390/ijms23031195.
3
Plasmodium vivax Infection Alters Mitochondrial Metabolism in Human Monocytes.

本文引用的文献

1
Innate immune recognition of an AT-rich stem-loop DNA motif in the Plasmodium falciparum genome.先天免疫识别恶性疟原虫基因组中富含 AT 的茎环 DNA 基序。
Immunity. 2011 Aug 26;35(2):194-207. doi: 10.1016/j.immuni.2011.05.016. Epub 2011 Aug 4.
2
Plasmodium vivax malaria: is it actually benign?间日疟原虫疟疾：真的良性吗？
J Infect Public Health. 2011 Jun;4(2):91-5. doi: 10.1016/j.jiph.2011.03.002. Epub 2011 May 26.
3
Divergent role of heme oxygenase inhibition in the pathogenesis of sepsis.血红素加氧酶抑制在脓毒症发病机制中的不同作用。
疟原虫感染改变人单核细胞中线粒体代谢。
mBio. 2021 Aug 31;12(4):e0124721. doi: 10.1128/mBio.01247-21. Epub 2021 Jul 27.
4
Factors influencing phagocytosis of malaria parasites: the story so far.影响疟原虫吞噬作用的因素：迄今为止的研究进展。
Malar J. 2021 Jul 16;20(1):319. doi: 10.1186/s12936-021-03849-1.
5
The GRKs Reactome: Role in Cell Biology and Pathology.GRKs 反应组：在细胞生物学和病理学中的作用。
Int J Mol Sci. 2021 Mar 25;22(7):3375. doi: 10.3390/ijms22073375.
6
The Interface Between Inflammatory Mediators and MicroRNAs in Severe Thrombocytopenia.炎症介质与严重血小板减少症中 microRNAs 的相互作用。
Front Cell Infect Microbiol. 2021 Mar 15;11:631333. doi: 10.3389/fcimb.2021.631333. eCollection 2021.
7
Impairs Antibacterial Innate Immunity to Systemic Infections in Part Through Hemozoin-Bound Bioactive Molecules.部分通过与疟原虫色素结合的生物活性分子损害对全身感染的抗菌固有免疫。
Front Cell Infect Microbiol. 2020 Jun 30;10:328. doi: 10.3389/fcimb.2020.00328. eCollection 2020.
8
Reduction in total leukocytes in malaria patients compared to febrile controls: A systematic review and meta-analysis.疟疾患者的白细胞总数减少与发热对照组相比：系统评价和荟萃分析。
PLoS One. 2020 Jun 23;15(6):e0233913. doi: 10.1371/journal.pone.0233913. eCollection 2020.
9
Plasma Proteins and Platelets Modulate Neutrophil Clearance of Malaria-Related Hemozoin Crystals.血浆蛋白和血小板调节中性粒细胞清除疟原虫相关血红素晶体。
Cells. 2019 Dec 30;9(1):93. doi: 10.3390/cells9010093.
10
Innate immunity to malaria-The role of monocytes.先天性疟疾免疫-单核细胞的作用。
Immunol Rev. 2020 Jan;293(1):8-24. doi: 10.1111/imr.12830. Epub 2019 Dec 16.
Shock. 2011 Jun;35(6):550-9. doi: 10.1097/SHK.0b013e31820e1ef0.
4
Therapeutical targeting of nucleic acid-sensing Toll-like receptors prevents experimental cerebral malaria.核酸感应 Toll 样受体治疗靶向预防实验性脑型疟疾。
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3689-94. doi: 10.1073/pnas.1015406108. Epub 2011 Feb 8.
5
Defying malaria: Fathoming severe Plasmodium vivax disease.对抗疟疾：深入了解间日疟原虫重症疾病
Nat Med. 2011 Jan;17(1):48-9. doi: 10.1038/nm0111-48.
6
Bacteremia in Kenyan children presenting with malaria.肯尼亚疟疾患儿的菌血症。
J Clin Microbiol. 2011 Feb;49(2):671-6. doi: 10.1128/JCM.01864-10. Epub 2010 Nov 24.
7
Severe Plasmodium vivax malaria, Brazilian Amazon.严重的间日疟原虫疟疾，巴西亚马孙地区。
Emerg Infect Dis. 2010 Oct;16(10):1611-4. doi: 10.3201/eid1610.100685.
8
CD4+ CD25+ Foxp3+ regulatory T cells, dendritic cells, and circulating cytokines in uncomplicated malaria: do different parasite species elicit similar host responses?CD4+ CD25+ Foxp3+ 调节性 T 细胞、树突状细胞和循环细胞因子在无并发症疟疾中的变化：不同疟原虫种类是否引起相似的宿主反应？
Infect Immun. 2010 Nov;78(11):4763-72. doi: 10.1128/IAI.00578-10. Epub 2010 Aug 16.
9
Clinical protection from falciparum malaria correlates with neutrophil respiratory bursts induced by merozoites opsonized with human serum antibodies.临床对恶性疟原虫的保护作用与被人血清抗体调理的疟原虫裂殖子诱导的中性粒细胞呼吸爆发有关。
PLoS One. 2010 Mar 25;5(3):e9871. doi: 10.1371/journal.pone.0009871.
10
Pattern recognition receptors and inflammation.模式识别受体与炎症。
Cell. 2010 Mar 19;140(6):805-20. doi: 10.1016/j.cell.2010.01.022.