实验室和野生鼠中小鼠诺如病毒的分子检测。
Molecular detection of murine noroviruses in laboratory and wild mice.
机构信息
Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
出版信息
Vet Microbiol. 2012 Dec 7;160(3-4):463-7. doi: 10.1016/j.vetmic.2012.06.002. Epub 2012 Jun 13.
Fecal specimens collected from 121 laboratory mice, 30 striped field mice (Apodemus agrarius), 70 yellow-necked mice (Apodemus flavicollis), and 3 bank voles (Myodes glareolus) were tested in sample pools for the presence of murine noroviruses (MNV). Ten of 41 laboratory mice and 2 of 3 striped field mice pooled samples were positive for MNV. All laboratory mouse MNVs were closely related to previously described MNVs. The complete ORF2 (VP1) of both striped field mouse MNVs identified in this study was 1623 nt (541 aa) long and differed at 12% nt (8% aa) positions from each other, at 22-24% nt (15-18% aa) positions from the laboratory mouse MNVs and at 20-22% nt (13-14% aa) positions from the recently described wood mouse (Apodemus sylvaticus) MNVs. This study provides further evidence for the circulation of novel, genetically diverse MNVs in wild mice.
从 121 只实验鼠、30 只黄胸鼠（Apodemus agrarius）、70 只黑线姬鼠（Apodemus flavicollis）和 3 只布氏田鼠（Myodes glareolus）中采集的粪便样本在样本池中进行了检测，以确定是否存在鼠诺如病毒（MNV）。41 只实验鼠中有 10 只和 3 只黄胸鼠的样本池呈 MNV 阳性。所有实验鼠的 MNV 都与先前描述的 MNV 密切相关。本研究中鉴定的两种黄胸鼠 MNV 的完整 ORF2（VP1）长 1623 个核苷酸（541 个氨基酸），彼此之间在 12%的核苷酸（8%的氨基酸）位置存在差异，与实验鼠 MNV 相比，在 22-24%的核苷酸（15-18%的氨基酸）位置存在差异，与最近描述的林鼠（Apodemus sylvaticus）MNV 相比，在 20-22%的核苷酸（13-14%的氨基酸）位置存在差异。本研究进一步证明了新型、遗传多样的 MNV 在野生鼠中的传播。
Zotero 插件