vTI-Institute of Agricultural Technology and Biosystems Engineering, Bundesallee 50, 38116, Braunschweig, Germany.
Appl Microbiol Biotechnol. 2012 Dec;96(5):1209-16. doi: 10.1007/s00253-012-4221-y. Epub 2012 Jul 1.
Biotechnologically produced itaconic acid (IA) is a promising organic acid with a wide range of applications and the potential to open up new application fields in the area of polymer chemistry, pharmacy, and agriculture. In this study, a systematic process optimization was performed with an own isolated strain of Aspergillus terreus and transferred from a 250-mL to a 15-L scale. An IA concentration of 86.2 g/L was achieved within 7 days with an overall productivity of 0.51 g/(L h), a maximum productivity of 1.2 g/(L h), and a yield of 86 mol%. A cultivation of other well-known A. terreus strains with the developed process showed no significant differences. Based on this, a process is developed providing a high final IA concentration independent of the used strain combined with high reproducibility.
生物技术生产的衣康酸（IA）是一种很有前途的有机酸，具有广泛的应用，并有潜力在聚合物化学、制药和农业领域开辟新的应用领域。在这项研究中，我们使用一株自行分离的土曲霉进行了系统的工艺优化，并将其从 250 毫升规模转移到 15 升规模。在 7 天内实现了 86.2 g/L 的 IA 浓度，总产率为 0.51 g/（L·h），最大产率为 1.2 g/（L·h），产率为 86 mol%。用开发的工艺对其他知名的土曲霉菌株进行培养，没有显示出显著的差异。在此基础上，开发了一种工艺，提供了一种高的最终 IA 浓度，不依赖于所用菌株，并具有高的重现性。
