Yi Xiunan, Alper Hal S
Interdisciplinary Life Sciences, The University of Texas at Austin, Austin, TX 78712, USA.
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
Life (Basel). 2022 Mar 30;12(4):510. doi: 10.3390/life12040510.
A variety of yeast species have been considered ideal hosts for metabolic engineering to produce value-added chemicals, including the model organism , as well as non-conventional yeasts including , , and . However, the metabolic capacity of these microbes is not simply dictated or implied by genus or species alone. Within the same species, yeast strains can display distinct variations in their phenotypes and metabolism, which affect the performance of introduced pathways and the production of interesting compounds. Moreover, it is unclear how this metabolic potential corresponds to function upon rewiring these organisms. These reports thus point out a new consideration for successful metabolic engineering, specifically: what are the best strains to utilize and how does one achieve effective metabolic engineering? Understanding such questions will accelerate the host selection and optimization process for generating yeast cell factories. In this review, we survey recent advances in studying yeast strain variations and utilizing non-type strains in pathway production and metabolic engineering applications. Additionally, we highlight the importance of employing portable methods for metabolic rewiring to best access this metabolic diversity. Finally, we conclude by highlighting the importance of considering strain diversity in metabolic engineering applications.
多种酵母物种被认为是代谢工程生产增值化学品的理想宿主，包括模式生物，以及非常规酵母，如、和。然而，这些微生物的代谢能力并非仅由属或种简单决定或暗示。在同一物种内，酵母菌株在其表型和代谢方面可表现出明显差异，这会影响引入途径的性能和目标化合物的生产。此外，尚不清楚这种代谢潜力在对这些生物体进行代谢重编程后如何与功能相对应。因此，这些报告指出了成功进行代谢工程的一个新考量，具体而言：使用哪些最佳菌株以及如何实现有效的代谢工程？理解这些问题将加速酵母细胞工厂宿主选择和优化过程。在本综述中，我们概述了研究酵母菌株变异以及在途径生产和代谢工程应用中利用非模式菌株的最新进展。此外，我们强调采用便于操作的方法进行代谢重编程以充分利用这种代谢多样性的重要性。最后，我们强调在代谢工程应用中考虑菌株多样性的重要性作为总结。
