Rizwan Komal, Zubair Muhammad, Rasool Nasir, Riaz Muhammad, Zia-Ul-Haq Muhammad, De Feo Vincenzo
Department of Chemistry, Government College University, Faisalabad 38000, Pakistan.
Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Karachi, Karachi 75270, Pakistan.
Int J Mol Sci. 2012;13(5):6440-6451. doi: 10.3390/ijms13056440. Epub 2012 May 24.
The present study was conducted to examine various biological activities of a methanol extract of Agave attenuata leaves. GC-MS analysis of the n-hexane fraction from the extract revealed the presence of 31 compounds, with mono-2-ethylhexyl phthalate (11.37%), 1,2-benzenedicarboxylic acid (6.33%), n-docosane (6.30%) and eicosane (6.02%) as the major components. The leaves contained appreciable levels of total phenolic contents (10.541-39.35 GAE, mg/100 g) and total flavonoid contents (43.35-304.8 CE, mg/100 g). The extract and some of its fractions showed moderate antimicrobial effects. Leaves extract and fractions also exhibited a good antioxidant potential when measured by DPPH radical scavenging activity and inhibition of lipid peroxidation assays. The hemolytic effect of the plant was found to be in a range of 1.01%-2.64%. From the present study it is concluded that this plant could be used as a source of natural antioxidants and functional food nutraceutical applications.
本研究旨在检测龙舌兰叶片甲醇提取物的各种生物活性。提取物正己烷馏分的气相色谱-质谱分析显示存在31种化合物，其中邻苯二甲酸单-2-乙基己酯（11.37%）、1,2-苯二甲酸（6.33%）、正二十二烷（6.30%）和正二十烷（6.02%）为主要成分。叶片中总酚含量（10.541 - 39.35 GAE，mg/100 g）和总黄酮含量（43.35 - 304.8 CE，mg/100 g）较高。提取物及其部分馏分显示出中等程度的抗菌作用。通过DPPH自由基清除活性和脂质过氧化抑制试验测定，叶片提取物及其馏分也表现出良好的抗氧化潜力。发现该植物的溶血作用在1.01% - 2.64%范围内。从本研究得出结论，这种植物可作为天然抗氧化剂的来源以及功能性食品营养保健品应用。
