一种诊断性单克隆免疫荧光试剂未能在环境样本中检测到嗜肺军团菌。
Failure of a diagnostic monoclonal immunofluorescent reagent to detect Legionella pneumophila in environmental samples.
作者信息
Vickers R M, Stout J E, Yu V L
机构信息
Special Pathogens Section, Veterans Administration Medical Center, Pittsburgh, Pennsylvania.
出版信息
Appl Environ Microbiol. 1990 Sep;56(9):2912-4. doi: 10.1128/aem.56.9.2912-2914.1990.
Three commercial diagnostic fluorescein-labeled antibodies, one monoclonal and two polyclonal, were compared to evaluate their abilities to detect Legionella pneumophila in environmental samples. The monoclonal conjugate failed to detect L. pneumophila in the 12 environmental samples studied by direct immunofluorescence. In contrast, the two polyclonal conjugates detected L. pneumophila in all 12 samples by both direct and indirect immunofluorescence. However, isolates recovered by culture from the 12 samples demonstrated equal immunofluorescence with all three conjugates. The reason for the failure of the monoclonal antibody to detect L. pneumophila in the environmental samples remains unknown. Laboratories considering the use of the monoclonal conjugate to screen environmental samples for L. pneumophila should be aware of this finding.
比较了三种商业诊断用荧光素标记抗体（一种单克隆抗体和两种多克隆抗体）检测环境样本中嗜肺军团菌的能力。通过直接免疫荧光法，单克隆偶联物未能在12份研究的环境样本中检测到嗜肺军团菌。相比之下，两种多克隆偶联物通过直接和间接免疫荧光法在所有12份样本中均检测到了嗜肺军团菌。然而，从这12份样本中通过培养回收的分离株与所有三种偶联物的免疫荧光反应相同。单克隆抗体未能在环境样本中检测到嗜肺军团菌的原因尚不清楚。考虑使用单克隆偶联物筛选环境样本中嗜肺军团菌的实验室应知晓这一发现。
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