采用DNA菌落杂交法检测溶血性单核细胞增生李斯特菌。
Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.
作者信息
Datta A R, Wentz B A, Hill W E
机构信息
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
出版信息
Appl Environ Microbiol. 1987 Sep;53(9):2256-9. doi: 10.1128/aem.53.9.2256-2259.1987.
Abstract
A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.
摘要
利用来自单核细胞增生李斯特菌的约500个碱基对的β-溶血素基因片段，通过DNA菌落杂交筛选不同的细菌菌株。在氢氧化钠存在的情况下，通过微波裂解菌落中的细胞。在筛选的52种不同李斯特菌菌株中，只有来自单核细胞增生李斯特菌β-溶血（CAMP阳性）菌株的DNA与该探针杂交。
Zotero 插件