Division of Periodontology, College of Dentistry, The Ohio State University, 305 W. 12th Ave., 4111 Postle Hall, Columbus, OH 43210, USA.
J Dent Res. 2010 Nov;89(11):1247-53. doi: 10.1177/0022034510377203. Epub 2010 Aug 25.
The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campylobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile of smoking-associated periodontitis is distinct from that of non-smokers, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.
龈下微生物群主要是未培养的，因此，基于培养和靶向分子的方法在检查吸烟对该群落的影响方面价值有限。我们通过使用开放式分子方法进行细菌鉴定来检验假设，即龈下生物膜在当前吸烟者和从不吸烟者中的组成不同。对当前吸烟者和从不吸烟者的深牙周袋菌斑进行匹配疾病的 16S 测序分析。吸烟者的 Parvimonas、Fusobacterium、Campylobacter、Bacteroides 和 Treponema 丰度更高，而 Veillonella、Neisseria 和 Streptococcus 的丰度更低。该组中几种未培养的 Peptostreptococci、Parvimonas micra、Campylobacter gracilis、Treponema socranskii、Dialister pneumosintes 和 Tannerella forsythia 升高，而 Veillonella sp. oral clone B2、Neisseria sp. oral clone 2.24、Streptococcus sanguinis 和 Capnocytophaga sp. clone AH015 的水平较低。与吸烟相关的牙周炎的微生物特征与不吸烟者不同，与疾病相关和与健康相容的生物体的流行率和丰度存在显著差异。
