Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Food Science, Purdue University, West Lafayette, 47907, IN, USA; Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, 47907, IN, USA.
Mol Cell Probes. 2020 Dec;54:101662. doi: 10.1016/j.mcp.2020.101662. Epub 2020 Sep 8.
The ongoing crisis due to the global pandemic caused by a highly contagious coronavirus (Coronavirus disease - 2019; COVID-19) and the lack of either proven effective therapy or a vaccine has made diagnostic a valuable tool in disease tracking and prevention. The complex nature of this newly emerging virus calls for scientists' attention to find the most reliable, highly sensitive, and selective detection techniques for better control or spread of the disease. Reverse transcriptase-polymerase chain reaction (RT-PCR) and serology-based tests are currently being used. However, the speed and accuracy of these tests may not meet the current demand; thus, alternative technology platforms are being developed. Nano biosensor technology platforms have been established as a promising diagnostic tool for rapid and accurate detection of viruses as well as other life-threatening diseases even in resource-limited settings. This review aims to provide a short overview of recent advancements in molecular and biosensor-based diagnosis of viruses, including the human coronaviruses, and highlight the challenges and future perspectives of these detection technologies.
由于高度传染性的冠状病毒（冠状病毒病-2019；COVID-19）引起的全球大流行而持续存在的危机，以及缺乏经过证实的有效治疗方法或疫苗，诊断已成为疾病跟踪和预防的有价值的工具。这种新出现的病毒的复杂性要求科学家们关注寻找最可靠、高度敏感和选择性的检测技术，以更好地控制或传播疾病。目前正在使用逆转录-聚合酶链反应（RT-PCR）和基于血清学的检测方法。然而，这些检测方法的速度和准确性可能无法满足当前的需求；因此，正在开发替代技术平台。纳米生物传感器技术平台已成为一种很有前途的诊断工具，可用于快速准确地检测病毒以及其他危及生命的疾病，即使在资源有限的环境中也是如此。本文旨在简要概述基于分子和生物传感器的病毒诊断的最新进展，包括人类冠状病毒，并强调这些检测技术的挑战和未来展望。
