Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge CB3 0FA, UK.
Sensors (Basel). 2024 Jan 28;24(3):856. doi: 10.3390/s24030856.
This paper presents the development of a miniaturized sensor device for selective detection of pathogens, specifically Influenza A Influenza virus, as an enveloped virus is relatively vulnerable to damaging environmental impacts. In consideration of environmental factors such as humidity and temperature, this particular pathogen proves to be an ideal choice for our study. It falls into the category of pathogens that pose greater challenges due to their susceptibility. An impedance biosensor was integrated into an existing platform and effectively separated and detected high concentrations of airborne pathogens. Bio-functionalized hydrogel-based detectors were utilized to analyze virus-containing particles. The sensor device demonstrated high sensitivity and specificity when exposed to varying concentrations of Influenza A virus ranging from 0.5 to 50 μg/mL. The sensitivity of the device for a 0.5 μg/mL analyte concentration was measured to be 695 Ω· mL/μg. Integration of this pathogen detector into a compact-design air quality monitoring device could foster the advancement of personal exposure monitoring applications. The proposed sensor device offers a promising approach for real-time pathogen detection in complex environmental settings.
本文提出了一种用于选择性检测病原体（特别是甲型流感病毒）的微型传感器设备的开发。由于包膜病毒相对容易受到环境影响的破坏，因此作为一种包膜病毒，它被选作我们研究的理想对象。它属于由于易感性而构成更大挑战的病原体类别之一。阻抗生物传感器被集成到现有平台中，并有效地分离和检测高浓度的空气传播病原体。基于生物功能化水凝胶的探测器用于分析含有病毒的颗粒。当暴露于浓度从 0.5 到 50μg/mL 变化的甲型流感病毒时，该传感器设备表现出高灵敏度和特异性。对于 0.5μg/mL 分析物浓度，该设备的灵敏度测量值为 695 Ω·mL/μg。将这种病原体探测器集成到紧凑型空气质量监测设备中，可以促进个人暴露监测应用的发展。所提出的传感器设备为在复杂的环境设置中进行实时病原体检测提供了一种有前途的方法。
